Laboratório de Inflamação, Metabolismo e Exercício (LAPIMEX) e Laboratório de Fisiologia Celular, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90035-003, RS, Brazil.
Programa de Pós-Graduação em Ciências do Movimento Humano, Escola de Educação Física, Fisioterapia e Dança (ESEFID), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90690-200, RS, Brazil.
Nutrients. 2024 Oct 18;16(20):3529. doi: 10.3390/nu16203529.
Physical inactivity induces insulin resistance (IR) and metabolic imbalances before any significant changes in adiposity. Recent studies suggest that the beneficial effects of exercise can be potentiated if performed while fasting. This work aimed to compare the subacute effects of fed- and fasted-state single-bout exercise on biochemical parameters and cellular signaling in the metabolism. The animals were allocated into fed rest (FER), fasting rest (FAR), fed exercise (FEE), and fasting exercise (FAE) groups. The exercise protocol was a 30 min treadmill session at 60% of V˙O. The fasting groups fasted for 8 h before exercise and were killed after 12 h post-exercise. Soleus glycogen concentration increased only in the fasting groups, whereas the triglyceride (TGL) content increased in brown adipose tissue (BAT) and liver in the FAE. The FAE showed decreased plasma total cholesterol concentration compared withthe FAR group. Immunocontent of HSP70, SIRT1, UCP-1, and PGC1-α did not change in any tissue investigated. Our results indicate that physical exercise while fasting can have beneficial metabolic effects on sedentary animals. Remarkably, in the FAE group, there was a reduction in total plasma cholesterol and an increase in the capacity of BAT to metabolize and store nutrients in the form of TGLs.
体力活动在肥胖发生明显变化之前会引起胰岛素抵抗（IR）和代谢失衡。最近的研究表明，如果在禁食状态下进行运动，运动的有益效果可以增强。本研究旨在比较进食和禁食状态下单次运动对代谢中生化参数和细胞信号的亚急性影响。动物被分为进食休息（FER）、禁食休息（FAR）、进食运动（FEE）和禁食运动（FAE）组。运动方案是在 60% V˙O 的跑步机上进行 30 分钟。禁食组在运动前禁食 8 小时，运动后 12 小时处死。仅在禁食组中，比目鱼肌糖原浓度增加，而在 FAE 中，棕色脂肪组织（BAT）和肝脏中的甘油三酯（TGL）含量增加。与 FAR 组相比，FAE 组的血浆总胆固醇浓度降低。在研究的任何组织中，HSP70、SIRT1、UCP-1 和 PGC1-α 的免疫含量均未发生变化。我们的结果表明，禁食状态下的体育锻炼对久坐不动的动物有有益的代谢作用。值得注意的是，在 FAE 组中，总血浆胆固醇减少，BAT 代谢和储存 TGL 等形式的营养物质的能力增加。
