Abadilla Janielle Mari S, Chen Bor-Yann, Ganzon Mike Anthony D, Caparanga Alvin R, Pamintuan Kristopher Ray S, Tayo Lemmuel L, Hsueh Chung-Chuan, Hsieh Cheng-Yang, Yang Ling-Ling, Tsai Po-Wei
School of Chemical, Biological, and Materials Engineering and Sciences, Mapúa University, Manila 1002, Philippines.
School of Graduate Studies, Mapúa University, Manila 1002, Philippines.
Plants (Basel). 2024 Oct 12;13(20):2857. doi: 10.3390/plants13202857.
L. (TA) pollen has been utilized as a traditional Chinese medicine for treating various internal and external traumas. Moreover, bioactive compounds possess diverse pharmacological activities. This study aims to evaluate the antiviral properties of TA based on its ability to generate bioenergy, capable of inhibiting viruses. TA pollens were extracted using water and ethanol solvents. These extracts were utilized to identify the phytochemical contents and correlate with the antioxidant activity via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. HPLC analysis was conducted to identify its electron-shuttling compositions. The bioenergy-generating characteristics were determined via microbial fuel cells. The water extract (TA-W) showed higher antioxidant activity due to a higher phenolic and flavonoid content compared to the ethanol extract (TA-E). Quercetin-3--(2-α-L-rhamnosyl)-rutinoside, quercetin-3--neohesperidoside, and quercetin are the electron shuttles (ES) identified out of the 11 compounds. TA obtained a 1.39 ± 0.10 amplification factor of power generation that indicates potential bioenergy-generating and associated antiviral characteristic properties. The findings may provide a foundation for developing antiviral medications specifically designed to target virus-related diseases, while minimizing the risk of drug toxicity and reducing the costs of drug development.
L.（TA）花粉一直被用作治疗各种内外伤的传统中药。此外，生物活性化合物具有多种药理活性。本研究旨在基于TA产生生物能的能力来评估其抗病毒特性，这种能力能够抑制病毒。TA花粉采用水和乙醇溶剂进行提取。这些提取物用于鉴定植物化学成分，并通过2,2 - 二苯基 - 1 - 苦基肼（DPPH）和铁还原抗氧化能力（FRAP）测定法与抗氧化活性相关联。进行高效液相色谱（HPLC）分析以鉴定其电子穿梭成分。通过微生物燃料电池测定生物能产生特性。与乙醇提取物（TA - E）相比，水提取物（TA - W）由于酚类和黄酮类含量较高而表现出更高的抗氧化活性。槲皮素 - 3 - [（2 - α - L - 鼠李糖基） - 芸香糖苷]、槲皮素 - 3 - 新橙皮糖苷和槲皮素是从11种化合物中鉴定出的电子穿梭体（ES）。TA获得了1.39±0.10的发电放大因子，表明其具有潜在的生物能产生及相关的抗病毒特性。这些发现可能为开发专门针对病毒相关疾病的抗病毒药物提供基础，同时将药物毒性风险降至最低并降低药物开发成本。
