Chen Peidong, Cao Yudan, Bao Beihua, Zhang Li, Ding Anwei
a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization , Nanjing University of Chinese Medicine , Nanjing , China.
Pharm Biol. 2017 Dec;55(1):1283-1288. doi: 10.1080/13880209.2017.1300818.
The pollen of Typha angustifolia L. (Typhaceae) has been used as a traditional Chinese medicine for improving the microcirculation and promoting wound healing. Flavonoids are the main constituent in the plant, but little is known about the antioxidant activity of the principal constituent of the pollen in detail.
To assess the antioxidant activities of ethanol and water extracts and two constituents of the pollen.
Plant material (1 g) was extracted by 95% ethanol and water (10 mL × 2, 1 h each), respectively. The extracted activities (0.8-2.6 mg/mL) were measured by DPPH and the reducing activity of ferric chloride (1.7-2.6 mg/mL). Typhaneoside and isorhamnetin-3-O-neohesperidoside (I3ON) (2.8-70 μmol/L) were investigated on the relationship between NO, MDA and SOD in HUVECs treated with 100 μg/mL of LPS for 24 h.
Nine compounds were identified by UPLC-MS. Ethanol extract showed IC values in DPPH (39.51 ± 0.72) and Fe reducing activity (82.76 ± 13.38), higher than the water extract (50.85 ± 0.74) and (106.33 ± 6.35), respectively. Typhaneoside and I3ON promoted cell proliferation at the respective concentration range of 2.8 to 70 μmol/L (p < 0.01). This two compounds decreased MDA (1.91 ± 0.10, 1.80 ± 0.34, p < 0.05) and NO levels (14.64 ± 0.08, 13.10 ± 0.88, p < 0.01), respectively, and increased SOD level (22.94 ± 2.48, 23.57 ± 2.38, p < 0.01) at the concentration of 70 μmol/L compared with LPS group.
The constituents from Typha angustifolia could be a novel therapeutic strategy for LPS-induced inflammation.
香蒲（香蒲科）花粉作为一种传统中药，具有改善微循环和促进伤口愈合的作用。黄酮类化合物是该植物的主要成分，但对花粉主要成分的抗氧化活性了解甚少。
评估香蒲花粉乙醇提取物、水提取物及其两种成分的抗氧化活性。
分别用95%乙醇和水（10 mL×2，每次1小时）提取1 g植物材料。提取物活性（0.8 - 2.6 mg/mL）通过DPPH法和氯化铁还原活性法（1.7 - 2.6 mg/mL）测定。研究了香蒲新苷和异鼠李素-3-O-新橙皮糖苷（I3ON）（2.8 - 70 μmol/L）对用100 μg/mL脂多糖处理24小时的人脐静脉内皮细胞（HUVECs）中一氧化氮（NO）、丙二醛（MDA）和超氧化物歧化酶（SOD）之间关系的影响。
通过超高效液相色谱-质谱联用（UPLC-MS）鉴定出9种化合物。乙醇提取物在DPPH法中的IC值（39.51±0.72）和铁还原活性（82.76±13.38），分别高于水提取物（50.85±0.74）和（106.33±6.35）。香蒲新苷和I3ON在各自2.8至70 μmol/L的浓度范围内促进细胞增殖（p<0.01）。与脂多糖组相比，这两种化合物在70 μmol/L浓度时分别降低了MDA水平（1.91±0.10，1.80±0.34，p<0.05）和NO水平（14.64±0.08，13.10±0.88，p<0.01），并提高了SOD水平（22.94±2.48，23.57±2.38，p<0.01）。
香蒲成分可能是脂多糖诱导炎症的一种新型治疗策略。
