School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Australia.
School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Australia; The Faculty of Biological Sciences, The University of Leeds, Leeds LS2 9JT, UK.
Food Res Int. 2022 Dec;162(Pt B):111951. doi: 10.1016/j.foodres.2022.111951. Epub 2022 Sep 19.
Polyphenols are vital bioactive constituents that have beneficial effects on human health. The aim of this study was to characterize the biologically active phenolic metabolites in Australian native commercial fruits (Kakadu plum, Davidson's plum, quandong peach, and muntries) and their antioxidant, α-glucosidase, and acetylcholinesterase inhibition activities. Polyphenols were measured through total phenolic content (TPC), total flavonoid content (TFC), total condensed tannin (TCT), and total monomeric anthocyanin content (TMAC). Moreover, different in-vitro biological assays (DPPH, ABTS, FICA, OH-RSA, α-glucosidase, and acetylcholinesterase inhibition activities) were conducted to measure the antioxidant, anti-diabetic, and anti-Alzheimer's potential of these selected fruits. LC-ESI-QTOF-MS/MS was implied for identification and quantification purposes. In this study, a total of 307 bioactive metabolites (51 phenolic acids, 194 flavonoids, 15 tannins, 23 other polyphenols, 5 stilbenes, 12 lignans, and 7 terpenoids) were putatively identified. A total of 41 phenolic compounds were quantified/semi-quantified. Kakadu plum was identified with a higher concentration of polyphenols and biological activities compared to Davidson plum, quandong peach, and muntries. Molecular docking was also conducted to discover the actual role of the most abundant phenolic metabolites in the α-glucosidase and acetylcholinesterase inhibition activities.
多酚是对人体健康有益的重要生物活性成分。本研究的目的是对澳大利亚本土商业水果（卡卡杜李、戴维森李、昆当桃和墨累李）中的生物活性酚类代谢物及其抗氧化、α-葡萄糖苷酶和乙酰胆碱酯酶抑制活性进行表征。多酚通过总酚含量（TPC）、总黄酮含量（TFC）、总缩合单宁（TCT）和总单体花青素含量（TMAC）进行测量。此外，还进行了不同的体外生物测定（DPPH、ABTS、FICA、OH-RSA、α-葡萄糖苷酶和乙酰胆碱酯酶抑制活性），以测量这些选定水果的抗氧化、抗糖尿病和抗阿尔茨海默病的潜力。LC-ESI-QTOF-MS/MS 用于鉴定和定量目的。在这项研究中，共鉴定出 307 种生物活性代谢物（51 种酚酸、194 种黄酮类化合物、15 种单宁、23 种其他多酚、5 种芪类、12 种木脂素和 7 种萜类化合物）。共定量/半定量了 41 种酚类化合物。与戴维森李、昆当桃和墨累李相比，卡卡杜李的多酚和生物活性浓度更高。还进行了分子对接，以发现最丰富的酚类代谢物在 α-葡萄糖苷酶和乙酰胆碱酯酶抑制活性中的实际作用。
