Hora Aline B, Biano Laiza S, Nascimento Ana Carla S, Camargo Zaine T, Heiden Greice I, Albulquerque-Júnior Ricardo L C, Grespan Renata, Aragão Jessica M D A, Camargo Enilton A
Graduate Program in Health Sciences, Federal University of Sergipe, São Cristóvão 49060-676, Brazil.
Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão 49107-230, Brazil.
Pharmaceuticals (Basel). 2024 Oct 14;17(10):1368. doi: 10.3390/ph17101368.
: Wound healing relies on a coordinated process with the participation of different mediators. Natural products are a source of active compounds with healing potential. Isoorientin is a natural flavone recognized as having several pharmacological properties, such as anti-inflammatory effects, making it a potential treatment for wounds. We investigated the effect of isoorientin on the healing of excisional skin wounds. : Male Swiss mice were subjected to the induction of excisional skin wounds (6 mm diameter) and treated with a vehicle (2% dimethyl sulfoxide in propylene glycol) or 2.5% isoorientin applied topically once a day for 14 days. The wound area was measured on days 0, 3, 7, and 14. Histopathological analyses were performed on the cicatricial tissue after 14 days. The myeloperoxidase activity and the interleukin-1β, tumoral necrosis factor (TNF)-α, and interleukin-6 concentrations were determined on the third day. : We observed that 3 days after the topical application of isoorientin, the lesion area was significantly smaller when compared to those of the vehicle ( < 0.01) and control ( < 0.05) groups. No difference was observed after 7 and 14 days of induction. Despite this, on day 14, histological analysis of cicatricial tissue from the animals treated with isoorientin showed reduced epidermal thickness ( < 0.001) and increased collagen deposition ( < 0.001). These effects were accompanied by decreased myeloperoxidase activity and interleukin-1β concentration on the third day of induction, without alteration in TNF-α and interleukin-6. : The treatment with isoorientin promoted better tissue repair in excisional wounds in mice, which may be linked to the modulation of the early inflammatory response.
伤口愈合依赖于一个有不同介质参与的协调过程。天然产物是具有愈合潜力的活性化合物的来源。异荭草素是一种天然黄酮，被认为具有多种药理特性，如抗炎作用，使其成为伤口的潜在治疗方法。我们研究了异荭草素对切除性皮肤伤口愈合的影响。雄性瑞士小鼠接受切除性皮肤伤口（直径6毫米）诱导，并每天一次局部涂抹赋形剂（丙二醇中2%的二甲基亚砜）或2.5%的异荭草素，持续14天。在第0、3、7和14天测量伤口面积。14天后对瘢痕组织进行组织病理学分析。在第三天测定髓过氧化物酶活性以及白细胞介素-1β、肿瘤坏死因子（TNF）-α和白细胞介素-6的浓度。我们观察到，局部应用异荭草素3天后，与赋形剂组（<0.01）和对照组（<0.05）相比，损伤面积显著更小。诱导7天和14天后未观察到差异。尽管如此，在第14天，对用异荭草素治疗的动物的瘢痕组织进行组织学分析显示表皮厚度降低（<0.001）和胶原沉积增加（<0.001）。这些效应伴随着诱导第三天髓过氧化物酶活性和白细胞介素-1β浓度降低，而TNF-α和白细胞介素-6没有改变。异荭草素治疗促进了小鼠切除伤口更好的组织修复，这可能与早期炎症反应的调节有关。
