Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Medicina (Kaunas). 2024 Oct 6;60(10):1632. doi: 10.3390/medicina60101632.
Middle East Respiratory Syndrome (MERS) is a viral respiratory illness caused by a coronavirus called Middle East respiratory syndrome. In the current study, immunoinformatics studies were applied to design an epitope-based vaccine construct against Middle East Respiratory Syndrome. In this study, epitopes base vaccine construct was designed against MERS using immunoinformatics approach. In this approach, the targeted proteins were screened, and probable antigenic, non-allergenic, and good water-soluble epitopes were selected for vaccine construction. In vaccine construction, the selected epitopes were joined by GPGPG linkers, and a linear multi-epitope vaccine was constructed. The vaccine construct underwent a physiochemical property analysis. The 3D structure of the vaccine construct was predicted and subjected to refinement. After the refinement, the 3D model was subjected to a molecular docking analysis, TLRs (TLR-3 and TLR-9) were selected as receptors for vaccine construct, and the molecular docking analysis study determined that the vaccine construct has binding ability with the targeted receptor. The docking analysis also unveils that the vaccine construct can properly activate immune system against the target virus however experimental validation is needed to confirm the in silico findings further.
中东呼吸综合征（MERS）是一种由一种名为中东呼吸综合征的冠状病毒引起的病毒性呼吸道疾病。在本研究中，应用免疫信息学研究设计了一种针对中东呼吸综合征的基于表位的疫苗构建体。 在这项研究中，使用免疫信息学方法设计了针对 MERS 的基于表位的疫苗构建体。 在这种方法中，筛选了靶向蛋白，并选择了可能的抗原性、非变应原性和良好水溶性的表位用于疫苗构建。在疫苗构建中，选择的表位通过 GPGPG 接头连接，并构建了线性多表位疫苗。对疫苗构建体进行了理化性质分析。预测了疫苗构建体的 3D 结构，并对其进行了细化。细化后，对 3D 模型进行了分子对接分析，选择 TLRs（TLR-3 和 TLR-9）作为疫苗构建体的受体，分子对接分析研究表明疫苗构建体具有与靶受体结合的能力。 对接分析还揭示了疫苗构建体可以适当地激活针对目标病毒的免疫系统，但需要进一步的实验验证来确认计算机模拟的结果。
