Molecular Biology and Interdisciplinary Life Sciences, New Mexico State University, Las Cruces, NM 88003, USA.
Microscopic Imaging Facility, New Mexico State University, Las Cruces, NM 88003, USA.
Viruses. 2024 Oct 5;16(10):1571. doi: 10.3390/v16101571.
Beet curly top virus (BCTV, , ) causes one of the most economically significant viral diseases in crops in the Western United States and is transmitted only by the beet leafhopper () in a non-propagative circulative manner. A better understanding of how this virus overcomes insect vector cellular barriers is essential to understanding virus-vector interactions. The distribution of BCTV in its beet leafhopper vector was investigated using immunofluorescence confocal laser scanning microscope analysis (iCLSM) on the whole-mount-dissected organs of leafhoppers. BCTV was localized in several lobes of the principal salivary glands, filter chamber, anterior midgut, and mid midgut, suggesting the occurrence of midgut and salivary gland barriers to BCTV transmission in its vector . This study also investigated the distribution of the chaperon GroEL homolog protein produced by primary endosymbiotic bacteria within the beet leafhopper, which is believed to indirectly affect viral transmission by enhancing insect immunity and resistance to viruses. GroEL was identified in leafhopper salivary glands lobes, the stylet, salivary canal, the filter chamber, and the Malpighian tubule. This is the first work to visualize the localization of a within its beet leafhopper vector. Together, these results can help understand ssDNA virus-vector relationships, including cellular transmission barriers and other vector protein components.
甜菜曲顶病毒（BCTV，）是美国西部作物中最重要的经济病毒病之一，只能通过叶蝉（）以非增殖循环方式传播。更好地了解这种病毒如何克服昆虫载体的细胞屏障对于理解病毒-载体相互作用至关重要。使用整置器官的免疫荧光共聚焦激光扫描显微镜分析（iCLSM）对叶蝉的全解剖器官进行了研究，以调查 BCTV 在其叶蝉载体中的分布。BCTV 定位于主要唾液腺的几个叶、过滤室、前中肠和中中肠，表明存在中肠和唾液腺屏障，阻止 BCTV 在其载体中传播。本研究还调查了初级内共生细菌产生的伴侣 GroEL 同源蛋白在甲虫叶蝉中的分布，据信它通过增强昆虫免疫力和对病毒的抵抗力来间接影响病毒的传播。GroEL 被鉴定存在于叶蝉唾液腺叶、喙、唾液道、过滤室和马氏管中。这是首次可视化其叶蝉载体中 ssDNA 病毒的定位。这些结果有助于理解 ssDNA 病毒-载体关系，包括细胞传播屏障和其他载体蛋白成分。
