Lemeshko V V, Babenko N A, Popova L Ia, Belostotskaia L I, Kozlova E V
Ukr Biokhim Zh (1978). 1986 Jan-Feb;58(1):63-8.
Rotation mobility of stearic acid spin labelling in mitochondria and microsomes of 24 months old rats is shown to decrease in comparison with that of 1 month rats. This decrease correlates with an increase in the free cholesterol content in membranes. The phospholipid composition is practically unchanged. With age the osmoresistance of organelles decreases and the cryoresistance increases, supporting indirectly the existence of structural changes in membranes with ageing. The most pronounced activation of NADPH-cytochrome C reductase in the microsomal membrane after the treatment with triton X-100 is observed in case of old animals. The thyroxine treatment of old rats causes a "rejuvenative" effect on the structural state of mitochondrial and microsomal membranes, evidencing for the important role of hormonal regulation in mechanisms of their age rearrangement.
与1月龄大鼠相比，24月龄大鼠线粒体和微粒体中硬脂酸自旋标记的旋转流动性降低。这种降低与膜中游离胆固醇含量的增加相关。磷脂组成实际上没有变化。随着年龄增长，细胞器的渗透压耐受性降低而抗冻性增加，这间接支持了膜随衰老发生结构变化的存在。在用曲拉通X-100处理后，老龄动物微粒体膜中NADPH-细胞色素C还原酶的激活最为明显。用甲状腺素处理老龄大鼠会对线粒体和微粒体膜的结构状态产生“返老还童”效应，这证明了激素调节在其年龄重排机制中的重要作用。
