Tianjin Medical University School and Hospital of Stomatology & Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, No. 12 Qixiangtai Road, Heping District, Tianjin 300070, PR China; Tianjin Medical University Institute of Stomatology, No. 12 Qixiangtai Road, Heping District, Tianjin 300070, PR China.
Tianjin Medical University School and Hospital of Stomatology & Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, No. 12 Qixiangtai Road, Heping District, Tianjin 300070, PR China; Tianjin Medical University Institute of Stomatology, No. 12 Qixiangtai Road, Heping District, Tianjin 300070, PR China; Department of Pediatric Dentistry, School and Hospital of Stomatology, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, Shandong 250012, China.
J Control Release. 2024 Dec;376:717-731. doi: 10.1016/j.jconrel.2024.10.041. Epub 2024 Oct 30.
Dental caries result from an imbalance between the demineralization and remineralization of dental hard tissues, primarily caused by biofilm accumulation. According to the theory of interrupting dental caries, effective anticaries materials and techniques should possess both remineralizing and antibacterial properties. However, current anticaries materials fail to mimic the process of amelogenesis to achieve remineralization while inhibiting the adhesion of cariogenic bacteria and the formation of biofilms. In this study, silk fibroin (SF) loaded with benzalkonium chloride (BZC) successfully formed an SF-BZC composite. This composite stabilized amorphous calcium phosphate (ACP), creating an ACP@SF-BZC dual-functional nanocomplex with both remineralizing and antibacterial properties. ACP@SF-BZC demonstrated significant anti-adhesion and biofilm inhibitory effects against Streptococcus mutans and Streptococcus sobrinus. Moreover, compared to fluoride, ACP@SF-BZC significantly enhanced the remineralization of demineralized enamel surfaces, forming a stable remineralized layer with improved mechanical properties, both in vitro and in vivo. In summary, the dual-function ACP@SF-BZC nanocomplex, with its remineralizing and antibacterial effects, offers a promising alternative for preventing and arresting enamel caries.
龋齿的发生是由于牙齿硬组织的脱矿和再矿化失衡所致，主要由生物膜的积聚引起。根据阻断龋齿理论，有效的防龋材料和技术应同时具有再矿化和抗菌性能。然而，目前的防龋材料无法模拟成釉过程来实现再矿化，同时抑制致龋菌的黏附和生物膜的形成。在本研究中，成功制备了载有苯扎氯铵（BZC）的丝素蛋白（SF）的 SF-BZC 复合材料。该复合材料稳定了无定形磷酸钙（ACP），形成了具有再矿化和抗菌功能的 ACP@SF-BZC 双功能纳米复合物。ACP@SF-BZC 对变形链球菌和远缘链球菌具有显著的抗黏附和抑制生物膜形成的作用。此外，与氟化物相比，ACP@SF-BZC 显著增强了脱矿釉质表面的再矿化作用，形成了具有改善的机械性能的稳定再矿化层，无论是在体外还是体内。综上所述，具有再矿化和抗菌作用的双功能 ACP@SF-BZC 纳米复合物为预防和控制釉质龋提供了一种有前途的替代方法。
