School of Stomatology, Hospital of Stomatology, Tianjin Medical University, Tianjin, China.
Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou, China.
J Dent Res. 2023 Nov;102(12):1315-1325. doi: 10.1177/00220345231189992. Epub 2023 Sep 12.
Dental caries is a dynamic disease induced by the unbalance between demineralization of dental hard tissues caused by biofilm and remineralization of them; however, although various effective remineralization methods have been well documented, it is a challenge to reestablish the balance by enhancing remineralization alone while ignoring the antibacterial therapy. Therefore, the integration of remineralizing and antibacterial technologies offers a promising strategy to halt natural caries progression in clinical practice. Here, the conception of interrupting dental caries (IDC) was proposed based on the development of dual-functional coating with remineralizing and antibacterial properties. In this study, bovine serum albumin (BSA) loaded octenidine (OCT) successfully to form a BSA-OCT composite. Subsequently, through fast amyloid-like aggregation, the phase-transited BSA-OCT (PTB-OCT) coating can be covered on teeth, resin composite, or sealant surfaces in 30 min by a simple smearing process. The PTB-OCT coating showed satisfactory effects in promoting the remineralization of demineralized enamel and dentin in vitro. Moreover, this coating also exerted significant acid-resistance stability and anti-biofilm properties. Equally importantly, this coating exhibited promising abilities in reducing the microleakage between the tooth and resin composite in vitro and preventing primary and secondary caries in vivo. In conclusion, this novel dual-functional PTB-OCT coating could reestablish the balance between demineralization and remineralization in the process of caries, thereby potentially preventing or arresting caries.
龋齿是一种由生物膜引起的牙体硬组织脱矿与再矿化失衡导致的动态疾病；然而，尽管各种有效的再矿化方法已有充分记载，但仅通过增强再矿化而忽略抗菌治疗来重新建立平衡仍然是一个挑战。因此，将再矿化和抗菌技术相结合为临床实践中阻止自然龋进展提供了一种很有前途的策略。基于具有再矿化和抗菌性能的双重功能涂层的发展，提出了阻断龋齿（IDC）的概念。在这项研究中，成功地将牛血清白蛋白（BSA）加载到奥替尼啶（OCT）中，形成 BSA-OCT 复合物。随后，通过快速类淀粉样聚集，PTB-OCT 涂层可以在 30 分钟内通过简单的涂抹过程覆盖在牙齿、树脂复合材料或密封剂表面上。PTB-OCT 涂层在促进体外脱矿釉质和牙本质再矿化方面表现出令人满意的效果。此外，这种涂层还具有显著的耐酸稳定性和抗生物膜性能。同样重要的是，这种涂层在减少体外牙齿和树脂复合材料之间的微渗漏以及预防原发性和继发性龋齿方面表现出良好的效果。总之，这种新型双重功能的 PTB-OCT 涂层可以在龋齿发生过程中重新建立脱矿与再矿化之间的平衡，从而可能预防或阻止龋齿的发生。
