School of Chemistry and Chemical Engineering, Institute of Environmental Science, Shanxi University, Taiyuan, 030006, China.
Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, 030032, China.
Mikrochim Acta. 2024 Oct 27;191(11):701. doi: 10.1007/s00604-024-06793-4.
A dual-responsive ratio electrochemical-colorimetric method for nitrite (NO) is established based on the combination of nanoenzyme (MnO) catalysis with diazotization reactions. The MnO can oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue TMBox. The NO induces the diazotization reaction of TMBox, leading to a decrease of the signal at 652 nm and the generation of a new signal from diazotized TMBox at 445 nm. Furthermore, the presence of NO reduces the electrochemical oxidation signal of TMB and simultaneously provides its electrochemical signal. Compared with traditional single-mode detection, dual-mode detection offers higher sensitivity, lower detection limits, and better interference resistance. The inherent advantages of this method make it feasible to detect NO in real samples, offering broad prospects for applications in food safety and environmental monitoring.
建立了一种基于纳米酶（MnO）催化与重氮化反应相结合的双响应比率电化学比色法用于检测亚硝酸根（NO）。MnO 可以将无色的 3,3',5,5'-四甲基联苯胺（TMB）氧化成蓝色 TMBox。NO 诱导 TMBox 的重氮化反应，导致在 652nm 处的信号降低，并且从重氮化的 TMBox 处生成新的信号在 445nm 处。此外，NO 的存在降低了 TMB 的电化学氧化信号，同时提供了其电化学信号。与传统的单模式检测相比，双模式检测具有更高的灵敏度、更低的检测限和更好的抗干扰能力。该方法的固有优势使其能够检测实际样品中的 NO，为食品安全和环境监测中的应用提供了广阔的前景。
