Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, 999 Phuttamonthon 4 Rd., Salaya, Phuttamonthon, Nakhon Pathom, 73170, Thailand.
Department of Biology, Faculty of Science, Mahidol University, Rama VI, Phayathai, Bangkok, 10400, Thailand.
Sci Rep. 2024 Oct 26;14(1):25510. doi: 10.1038/s41598-024-76428-4.
Pasteurella aerogenes has been implicated in reproductive disorders in sows, yet its prevalence and characteristics in vaginal discharge are not well understood. This study aimed to detect P. aerogenes in sow vaginal discharge samples and investigate its antibiotic resistance profile, toxin genes, and toxicity. P. aerogenes was isolated from 40% (8/20) of samples. Antimicrobial susceptibility testing revealed universal resistance to amoxicillin-clavulanate (4:1), with 87.5% of isolates also resistant to oxytetracycline, amoxicillin, ceftriaxone, and enrofloxacin. The colistin resistance gene mcr-2 was detected in 75% of isolates, while class 1 integron (int1) was found in 12.5%. The pax toxin gene cluster was present in 75% of isolates. Toxicity assays using Panagrellus redivivus demonstrated dose-dependent effects of P. aerogenes supernatant containing pax toxins. This study represents the first report of P. aerogenes isolation from sow vaginal discharge in Thailand. The high prevalence of antibiotic resistance, presence of the mcr-2 gene, and toxicity of pax toxin-positive isolates suggest that P. aerogenes may be an underestimated factor in swine reproductive health. These findings highlight the need for further investigation into the role of P. aerogenes in sow reproductive disorders and its potential impact on swine production.
多杀巴斯德氏菌已被牵连到母猪的生殖障碍中，但它在阴道分泌物中的流行情况和特征尚不清楚。本研究旨在检测母猪阴道分泌物样本中的多杀巴斯德氏菌，并研究其抗生素耐药谱、毒素基因和毒性。从 40%（8/20）的样本中分离出多杀巴斯德氏菌。药敏试验显示对阿莫西林-克拉维酸（4:1）普遍耐药，87.5%的分离株对土霉素、阿莫西林、头孢曲松和恩诺沙星也耐药。75%的分离株检测到黏菌素耐药基因 mcr-2，而 12.5%的分离株检测到类 1 整合子（int1）。75%的分离株存在 pax 毒素基因簇。使用潘格尔鲁斯雷迪维图斯进行的毒性试验表明，含有 pax 毒素的多杀巴斯德氏菌上清液具有剂量依赖性效应。本研究代表了泰国首次从母猪阴道分泌物中分离出多杀巴斯德氏菌的报告。高比例的抗生素耐药性、mcr-2 基因的存在以及产 pax 毒素阳性分离株的毒性表明，多杀巴斯德氏菌可能是猪生殖健康中被低估的因素。这些发现强调了需要进一步研究多杀巴斯德氏菌在母猪生殖障碍中的作用及其对养猪生产的潜在影响。
