Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
Bioscience and Biotechnology Center, Nagoya University, Nagoya, Japan.
Commun Biol. 2024 Oct 26;7(1):1396. doi: 10.1038/s42003-024-07102-8.
Membrane-less organelles, formed by the condensation of biomolecules, play a pivotal role in eukaryotes. Artificial membrane-less organelles and condensates are effective tools for the creation of new cellular functions. However, it is poorly understood how to control the properties that affect condensate function, particularly in plants. Here, we report the construction of model artificial condensates using the condensation-prone proteins OsJAZ2 and AtFCA in a transient assay using rice (Oryza sativa) cells, and how condensate properties, such as subcellular localization, protein mobility, and size can be altered. We showed that proteins of interest can be recruited to condensates using nanobodies or chemically induced dimerization. Furthermore, by combining two types of condensation-prone proteins, we demonstrated that artificial hybrid condensates with heterogeneous material properties could be constructed. Finally, we showed that modified artificial condensates can be constructed in transgenic Arabidopsis thaliana plants. These results provide a framework for the basic design of synthetic membrane-less organelles in plants.
无膜细胞器由生物分子的凝聚形成，在真核生物中发挥着关键作用。人工无膜细胞器和凝聚物是创造新细胞功能的有效工具。然而，人们对如何控制影响凝聚物功能的特性知之甚少，特别是在植物中。在这里，我们报告了使用易凝聚蛋白 OsJAZ2 和 AtFCA 在使用水稻（Oryza sativa）细胞的瞬时测定中构建模型人工凝聚物，以及如何改变凝聚物的特性，如亚细胞定位、蛋白质流动性和大小。我们表明，可以使用纳米体或化学诱导的二聚化将感兴趣的蛋白质募集到凝聚物中。此外，通过结合两种类型的易凝聚蛋白，我们证明可以构建具有异质材料特性的人工杂交凝聚物。最后，我们表明可以在转基因拟南芥（Arabidopsis thaliana）植物中构建修饰的人工凝聚物。这些结果为植物中合成无膜细胞器的基本设计提供了框架。
