Henan University of Chinese Medicine, Zhengzhou, China.
Department of Andrology, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, China.
Am J Mens Health. 2024 Sep-Oct;18(5):15579883241286672. doi: 10.1177/15579883241286672.
To analyze the differential expression profiles of microRNAs (miRNAs) in spermatozoa of patients with sperm DNA damage and to investigate the role of miRNAs in sperm DNA damage. Male infertility patients with sperm DNA damage who attended the First Affiliated Hospital of Henan University of Chinese Medicine from October 2023 to December 2023 were selected and included in this study as a case group. Fertile healthy men who were seen at the health check-up center during the same period and diagnosed by examination were also included as a control group. Sperm miRNA expression was detected in patients with sperm DNA damage (case group, = 5) and healthy medical check-ups (control group, = 5) using high-throughput sequencing technology. The differentially expressed miRNAs between the two groups were bioinformatically analyzed to explore the main biological functions of the target genes. We found that 63 miRNAs were significantly changed in the spermatozoa of patients with sperm DNA damage,|log2 (foldchange)| ≥ 1, < .05. Gene Ontology (GO) enrichment analysis indicated that these differential miRNAs might be involved in developmental process, anatomical structure development, cellular macromolecule metabolic process, multicellular organism development, system development, and so on. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that that they mainly affect the PI3K-AKT signaling pathway. The present study suggests that the altered expression of miR-1255a, miR-921, and miR-3156-5p may play an important role in the sperm DNA damage process, and the mechanism may involve the phosphatidylinositol-3'-kinase-AKT (PI3K-AKT) signaling pathway.
分析精子 DNA 损伤患者精子中 microRNAs (miRNAs) 的差异表达谱，并探讨 miRNAs 在精子 DNA 损伤中的作用。选择 2023 年 10 月至 12 月在河南中医药大学第一附属医院就诊的精子 DNA 损伤的男性不育患者作为病例组，并纳入研究。同时纳入同期在健康体检中心体检、经检查诊断为健康的男性作为对照组。采用高通量测序技术检测精子 DNA 损伤患者（病例组，n = 5）和健康体检者（对照组，n = 5）的精子 miRNA 表达。对两组间差异表达的 miRNAs 进行生物信息学分析，以探讨靶基因的主要生物学功能。结果发现，精子 DNA 损伤患者精子中 63 个 miRNAs 表达明显改变，|log2(foldchange)|≥1，P<0.05。基因本体论（GO）富集分析表明，这些差异表达的 miRNAs 可能参与发育过程、解剖结构发育、细胞大分子代谢过程、多细胞生物发育、系统发育等。京都基因与基因组百科全书（KEGG）富集分析显示，它们主要影响 PI3K-AKT 信号通路。本研究提示 miR-1255a、miR-921 和 miR-3156-5p 的表达改变可能在精子 DNA 损伤过程中发挥重要作用，其机制可能涉及磷脂酰肌醇-3'-激酶-AKT（PI3K-AKT）信号通路。
