Tadamura Kazuki, Torada Atsushi, Yoshiga Toyoshi
HOKUREN Agricultural Research Institute.
Laboratory of Nematology, Faculty of Agriculture, Saga University.
Plant Biotechnol (Tokyo). 2024 Jun 25;41(2):137-145. doi: 10.5511/plantbiotechnology.24.0428a.
is a plant-parasitic nematode that severely damages garlic ( L.) in Japan. is detected in roots, bulbs, and outer bulb skins of garlic at harvest; however, the resistance of garlic to infection is not well understood. Here, we investigated the propagation of in storage organs and roots using in vitro plantlets of six Japanese garlic varieties to exclude the effects of microbes and to uniform growing conditions. In vitro inoculation can proceed simultaneously with vegetative growth, storage organ formation of garlic plantlets, and infection. In 'Fukuchi-white', a variety susceptible to , nematodes successfully propagated in storage organs and roots. Furthermore, the nematodes invaded and propagated in the newly formed storage organs. By contrast, 'Kirishima', 'Hirado', and 'Shishimaru' substantially suppressed more the propagation of the nematodes in storage organs and roots than 'Fukuchi-white'. Additionally, the propagation of nematodes in newly formed storage organs was inhibited in these three varieties. 'Shishimaru' showed unique responses to infection: nematode propagation was the lowest among six varieties in inoculation tests and the nematode-inoculated cloves turned brown. Our results suggest that several garlic varieties have resistance mechanisms that suppress the propagation of in storage organs and roots, and that in vitro inoculation methods are useful for selecting resistant garlic varieties. These findings will help developing novel -resistant garlic varieties and our further understanding of garlic-nematode interactions.
是一种植物寄生线虫，在日本严重损害大蒜（L.）。在收获时，可在大蒜的根、鳞茎和鳞茎外皮中检测到。然而，大蒜对感染的抗性尚不清楚。在这里，我们使用六个日本大蒜品种的离体幼苗研究了该线虫在储存器官和根中的繁殖情况，以排除微生物的影响并使生长条件一致。离体接种可与大蒜幼苗的营养生长、储存器官形成以及线虫感染同时进行。在易感染该线虫的“福地白”品种中，线虫在储存器官和根中成功繁殖。此外，线虫侵入并在新形成的储存器官中繁殖。相比之下，“雾岛”、“平户”和“狮子丸”比“福地白”更能显著抑制线虫在储存器官和根中的繁殖。此外，在这三个品种中，线虫在新形成的储存器官中的繁殖受到抑制。“狮子丸”对该线虫感染表现出独特的反应：在接种试验中，线虫繁殖在六个品种中最低，且接种线虫的蒜瓣变成褐色。我们的结果表明，几个大蒜品种具有抑制线虫在储存器官和根中繁殖的抗性机制，并且离体接种方法有助于选择抗性大蒜品种。这些发现将有助于培育新型抗该线虫的大蒜品种，并加深我们对大蒜与线虫相互作用的进一步理解。
