Cremer Julia, Campbell Paul, Steele Visnja, Persley Denis, Thomas John, Harper Stephen, Gambley Cherie
Department of Agriculture and Fisheries, Brisbane, QLD 4001, Australia.
Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD 4001, Australia.
Plants (Basel). 2021 May 19;10(5):1013. doi: 10.3390/plants10051013.
The distribution of viruses in eastern Australian field garlic was evaluated. Detection assays were developed that involved generic RT-PCR for viruses in the , and genera followed by virus-specific colorimetric dot-blot hybridization. Assays targeted the potyviruses (onion yellow dwarf virus (OYDV), shallot yellow stripe virus (SYSV), and leek yellow stripe virus (LYSV)), the carlaviruses (garlic common latent virus (GCLV) and shallot latent virus (SLV)), and the allexiviruses (garlic viruses A, B, C, X (GarVA, -B, -C, -X) and shallot virus X (ShVX)). Virus incidence in crops was consistently high, with most plants infected with at least one virus from each genus. OYDV, LYSV, SLV, and GCLV were commonly detected. Three of the four allexiviruses were in all districts surveyed but varied in incidence, whereas ShVX and SYSV were not detected. There was no association between virus species complement and bulb size, indicating size is not a good predictor of the virus status of planting material. The variation of virus incidence across different Australian growing districts and in different cultivars implies multiple introductions of viruses rather than spread within the country. The genetic diversity observed within coat protein sequences of some virus species also supports multiple separate introductions.
对澳大利亚东部野蒜中的病毒分布进行了评估。开发了检测方法，包括针对 、 和 属病毒的通用逆转录聚合酶链反应（RT-PCR），随后进行病毒特异性比色斑点杂交。检测方法针对马铃薯Y病毒属（洋葱黄矮病毒（OYDV）、青葱黄条病毒（SYSV）和韭菜黄条病毒（LYSV））、香石竹潜隐病毒属（大蒜普通潜隐病毒（GCLV）和青葱潜隐病毒（SLV））以及大蒜病毒属（大蒜病毒A、B、C、X（GarVA、-B、-C、-X）和青葱病毒X（ShVX））。作物中的病毒发病率一直很高，大多数植株感染了每个属中至少一种病毒。OYDV、LYSV、SLV和GCLV经常被检测到。在所调查的所有地区均检测到四种大蒜病毒属病毒中的三种，但发病率有所不同，而未检测到ShVX和SYSV。病毒种类组合与鳞茎大小之间没有关联，这表明大小不是种植材料病毒状态的良好预测指标。澳大利亚不同种植区和不同品种间病毒发病率的差异意味着病毒是多次传入而非在国内传播。在一些病毒种类的外壳蛋白序列中观察到的遗传多样性也支持多次独立传入。
