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番茄中的一种小热激蛋白（SlHSP17.3）在盐胁迫中发挥着积极作用。

A small heat shock protein (SlHSP17.3) in tomato plays a positive role in salt stress.

作者信息

Cai Guohua, Niu Mingyu, Sun Zhihao, Wang Huakun, Zhang Shuo, Liu Fei, Wu Yanqun, Wang Guodong

机构信息

School of Biological Sciences, Jining Medical University, Rizhao, Shandong, China.

出版信息

Front Plant Sci. 2024 Oct 11;15:1443625. doi: 10.3389/fpls.2024.1443625. eCollection 2024.

DOI:10.3389/fpls.2024.1443625
PMID:39464285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11503465/
Abstract

Small heat shock proteins (sHSPs) are molecular chaperones that are widely present in plants and play a vital role in the response of plants to various environmental stimuli. This study employed transgenic to investigate the impact of the new tomato () sHSP protein (SlHSP17.3) on salt stress tolerance. Transient conversion analysis of protoplasts revealed that SlHSP17.3 localized to the cytoplasm. Furthermore, as suggested by expression analysis, salt stress stimulated expression, suggesting that SlHSP17.3 is involved in the salt stress response of plants. -overexpressing plants presented greater germination rates, fresh weights, chlorophyll contents, and Fv/Fm ratios, as well as longer root lengths, lower reactive oxygen species (ROS) levels, and lighter cell membrane injury under salt stress. Furthermore, certain stress-related genes (, , and ) were up-regulated in salt-stressed transgenic plants. Overall, overexpression improved the salt stress resistance of transgenic plants, mainly through increasing , , and expression.

摘要

小分子热激蛋白（sHSPs）是一类分子伴侣，广泛存在于植物中，在植物对各种环境刺激的响应中发挥着至关重要的作用。本研究采用转基因技术来探究新型番茄（）小分子热激蛋白（SlHSP17.3）对耐盐性的影响。对原生质体进行瞬时转化分析表明，SlHSP17.3定位于细胞质中。此外，表达分析显示，盐胁迫会刺激其表达，这表明SlHSP17.3参与植物的盐胁迫响应。过表达SlHSP17.3的植株在盐胁迫下表现出更高的发芽率、鲜重、叶绿素含量和Fv/Fm比值，以及更长的根长、更低的活性氧（ROS）水平和更轻的细胞膜损伤。此外，在盐胁迫的转基因植株中，某些与胁迫相关的基因（、和）上调表达。总体而言，SlHSP17.3过表达提高了转基因植株的耐盐性，主要是通过增加、和的表达来实现的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/a1caf6d4918a/fpls-15-1443625-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/f0cf8ae6e29f/fpls-15-1443625-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/0a96d0aa63f1/fpls-15-1443625-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/c42fa66d1cff/fpls-15-1443625-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/fcfd0ae53008/fpls-15-1443625-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/d5e5ede75106/fpls-15-1443625-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/e82b97138028/fpls-15-1443625-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/013203968870/fpls-15-1443625-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/0baa3fb00e47/fpls-15-1443625-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/67923e587df9/fpls-15-1443625-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/ce7602eb2748/fpls-15-1443625-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/a1caf6d4918a/fpls-15-1443625-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/f0cf8ae6e29f/fpls-15-1443625-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/0a96d0aa63f1/fpls-15-1443625-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/c42fa66d1cff/fpls-15-1443625-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/fcfd0ae53008/fpls-15-1443625-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/d5e5ede75106/fpls-15-1443625-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/e82b97138028/fpls-15-1443625-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/013203968870/fpls-15-1443625-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/0baa3fb00e47/fpls-15-1443625-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/67923e587df9/fpls-15-1443625-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/ce7602eb2748/fpls-15-1443625-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ba9/11503465/a1caf6d4918a/fpls-15-1443625-g011.jpg

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Plant Physiol Biochem. 2022 Apr 15;177:1-9. doi: 10.1016/j.plaphy.2022.02.017. Epub 2022 Feb 20.
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