Department of Plant Sciences, MIGAL - Galilee Research Institute , Kiryat-Shmona, Israel.
Plant Signal Behav. 2020 Aug 2;15(8):1776026. doi: 10.1080/15592324.2020.1776026. Epub 2020 Jun 21.
Earlier, we reported that Arabidopsis young lateral roots (LR) exhibited improved lethal salinity tolerance as compared with the primary root (PR). We have shown that cell death processes which take place in the PR during salt stress are postponed in the LR. Still, very little is known about the regulation of cell survival mechanisms in the LR during salinity stress. Here we used transgenic Arabidopsis plants expressing Cyclin B1;1:GUS, to further study the responses to salinity in the PR and LR positions. We found strong Cyclin B1;1:GUS activity in young budding LR of salt stressed and stress recovered plants. The Cyclin B1;1:GUS activity dropped significantly in long LR and was almost completely abolished in the PR. Our data provides another line of evidence that position-dependent response occurs in Arabidopsis roots during lethal salinity. The possible roles Cyclin B1;1 plays in the young LR during the response to lethal salinity are discussed.
早些时候，我们报道称与主根（PR）相比，拟南芥幼侧根（LR）表现出增强的致死盐胁迫耐受性。我们已经表明，在盐胁迫过程中发生在 PR 中的细胞死亡过程在 LR 中被推迟。尽管如此，关于在盐胁迫期间 LR 中细胞存活机制的调节，我们知之甚少。在这里，我们使用表达细胞周期蛋白 B1;1:GUS 的转基因拟南芥植物，进一步研究 PR 和 LR 位置对盐度的反应。我们发现，在受盐胁迫和应激恢复的植物的幼芽 LR 中，Cyclin B1;1:GUS 活性很强。Cyclin B1;1:GUS 活性在长 LR 中显著下降，在 PR 中几乎完全消失。我们的数据提供了另一个证据，表明在致死盐胁迫下，拟南芥根中存在位置依赖性反应。讨论了细胞周期蛋白 B1;1 在应对致死盐胁迫时在幼 LR 中可能发挥的作用。
