Richard D
Am J Physiol. 1986 Feb;250(2 Pt 2):R245-9. doi: 10.1152/ajpregu.1986.250.2.R245.
This study was carried out to investigate the nutritional energetics of ovariectomized rats with or without ovarian hormone replacement. Rats were divided into five groups: 1) sham operated, 2) ovariectomized, 3) ovariectomized and treated with progesterone, 4) ovariectomized and treated with estradiol, or 5) ovariectomized and treated with estradiol and progesterone. After 36 days of treatment, energy contents of all five groups were determined together with energy content of food and feces. Brown adipose tissue thermogenesis was assessed through mitochondrial GDP binding assay. Results show that ovariectomy leads to a 16% increase in metabolizable energy intake. This increase was accompanied by a twofold increase in body energy gain. Progesterone did not further affect energy intake and gain in ovariectomized rats. However, increases in both food intake and energy gain were prevented by the estradiol replacement therapy. There was no difference in energy expenditure between sham-operated and ovariectomized rats in the absence of estradiol. In estradiol-treated animals, energy expenditure (kJ.kg body wt-0.75 . day-1) showed a slight increase. There was no difference in protein content of interscapular brown adipose tissue between all five groups. GDP binding was slightly reduced in ovariectomized estradiol-treated rats. It is concluded from this study that ovarian hormones produce their effects on energy balance mainly by altering food intake.
本研究旨在调查去卵巢大鼠在有无卵巢激素替代情况下的营养能量学。大鼠被分为五组：1）假手术组，2）去卵巢组，3）去卵巢并用孕酮治疗组，4）去卵巢并用雌二醇治疗组，或5）去卵巢并用雌二醇和孕酮治疗组。治疗36天后，测定所有五组的能量含量以及食物和粪便的能量含量。通过线粒体GDP结合试验评估棕色脂肪组织产热。结果表明，去卵巢导致可代谢能量摄入增加16%。这种增加伴随着身体能量增加两倍。孕酮对去卵巢大鼠的能量摄入和增加没有进一步影响。然而，雌二醇替代疗法阻止了食物摄入量和能量增加的增加。在没有雌二醇的情况下，假手术组和去卵巢组之间的能量消耗没有差异。在接受雌二醇治疗的动物中，能量消耗（kJ·kg体重-0.75·天-1）略有增加。所有五组之间肩胛间棕色脂肪组织的蛋白质含量没有差异。在接受雌二醇治疗的去卵巢大鼠中，GDP结合略有降低。从这项研究得出的结论是，卵巢激素主要通过改变食物摄入量对能量平衡产生影响。
