Department of Neurosurgery, The First Medical Center of PLA General Hospital, Beijing, 100853, China.
Department of Orthopaedics, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
J Mater Sci Mater Med. 2024 Oct 28;35(1):66. doi: 10.1007/s10856-024-06835-6.
An acellular bovine bone matrix modified to release Magnesium ions (Mg) (ABBM-Mg) was prepared and evaluated for its potential in osteogenesis and mandibular defect repair. Mg was incorporated into ABBM using an ion exchange method. The microstructure and mechanical properties of both ABBM and ABBM-Mg were analyzed using SEM and a biomechanical testing machine. Cytocompatibility, cell adhesion, and osteogenic differentiation were assessed using various methods including CCK-8, Live/Dead staining, SEM, ALP staining, and qPCR analysis in MC3T3-E1 cells. Additionally, a mandibular defect model in rats was established. The bone defect repair outcomes were evaluated using Micro-CT, histological HE staining, and Masson staining. The study showed that mineralization containing magnesium was redeposited on the surface of the three-dimensional porous ABBM, and the ABBM-Mg scaffold promoted cell proliferation and osteogenic differentiation compared to the ABBM scaffold. In the rat mandibular defect model, the ABBM-Mg scaffold demonstrated superior bone repair ability. This study successfully incorporated Mg into ABBM without significantly affecting its microstructure and compressive strength. Furthermore, ABBM-Mg showed sustained release of Mg which enhanced cell proliferation, adhesion, and osteogenic differentiation in vitro, and promoted mandibular defect healing in rats. This research opens up new possibilities for the clinical application of functionalized acellular bone matrix.
一种经处理可释放镁离子（Mg）的脱细胞牛骨基质（ABBM-Mg）被制备并评估其在成骨和下颌骨缺损修复中的潜力。通过离子交换法将 Mg 掺入 ABBM 中。使用 SEM 和生物力学试验机分析了 ABBM 和 ABBM-Mg 的微观结构和力学性能。通过 CCK-8、Live/Dead 染色、SEM、ALP 染色和 qPCR 分析等方法评估了 MC3T3-E1 细胞的细胞相容性、细胞黏附和成骨分化。此外，还建立了大鼠下颌骨缺损模型。使用 Micro-CT、组织学 HE 染色和 Masson 染色评估骨缺损修复结果。研究表明，含镁的矿物质重新沉积在三维多孔 ABBM 的表面上，与 ABBM 支架相比，ABBM-Mg 支架促进了细胞增殖和成骨分化。在大鼠下颌骨缺损模型中，ABBM-Mg 支架表现出优异的骨修复能力。本研究成功地将 Mg 掺入 ABBM 中，而不会显著影响其微观结构和压缩强度。此外，ABBM-Mg 表现出持续释放 Mg，这增强了细胞增殖、黏附和体外成骨分化，并促进了大鼠下颌骨缺损的愈合。这项研究为功能化脱细胞骨基质的临床应用开辟了新的可能性。
