Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig 23119, Turkey.
Department of Nuclear Medicine, School of Medicine, Firat University, Elazig 23119, Turkey.
Nutrients. 2021 Sep 24;13(10):3353. doi: 10.3390/nu13103353.
Magnesium (Mg) deficiency may affect bone metabolism by increasing osteoclasts, decreasing osteoblasts, promoting inflammation/oxidative stress, and result in subsequent bone loss. The objective of the present study was to identify the molecular mechanism underlying the bone protective effect of different forms of Mg (inorganic magnesium oxide (MgO) versus organic magnesium picolinate (MgPic) compound) in rats fed with a high-fat diet (HFD). Forty-two Wistar albino male rats were divided into six group ( = 7): (i) control, (ii) MgO, (iii) MgPic, (iv) HFD, (v) HFD + MgO, and (vi) HFD + MgPic. Bone mineral density (BMD) increased in the Mg supplemented groups, especially MgPic, as compared with the HFD group ( 0.001). As compared with the HFD + MgO group, the HFD + MgPic group had higher bone P ( < 0.05) and Mg levels ( 0.001). In addition, as compared to MgO, MgPic improved bone formation by increasing the levels of osteogenetic proteins (COL1A1 ( 0.001), BMP2 ( 0.001), Runx2 ( 0.001), OPG ( < 0.05), and OCN ( < 0.001), IGF-1 ( < 0.001)), while prevented bone resorption by reducing the levels of RANK and RANKL ( < 0.001). In conclusion, the present data showed that the MgPic could increase osteogenic protein levels in bone more effectively than MgO, prevent bone loss, and contribute to bone formation in HFD rats.
镁（Mg）缺乏可通过增加破骨细胞、减少成骨细胞、促进炎症/氧化应激来影响骨代谢，从而导致随后的骨丢失。本研究的目的是确定不同形式的镁（无机氧化镁（MgO）与有机镁吡啶酸酯（MgPic）复合物）对高脂肪饮食（HFD）喂养大鼠的骨保护作用的分子机制。将 42 只 Wistar 白化雄性大鼠分为六组（n = 7）：（i）对照组，（ii）MgO，（iii）MgPic，（iv）HFD，（v）HFD + MgO，和（vi）HFD + MgPic。与 HFD 组相比，补充镁的组（尤其是 MgPic）的骨矿物质密度（BMD）增加（p < 0.001）。与 HFD + MgO 组相比，HFD + MgPic 组的骨磷（p < 0.05）和镁水平更高（p < 0.001）。此外，与 MgO 相比，MgPic 通过增加成骨蛋白水平来改善骨形成（COL1A1（p < 0.001）、BMP2（p < 0.001）、Runx2（p < 0.001）、OPG（p < 0.05）和 OCN（p < 0.001）），同时通过降低 RANK 和 RANKL 水平来抑制骨吸收（p < 0.001）。总之，本研究数据表明，MgPic 比 MgO 更能有效地增加骨中成骨蛋白水平，防止骨丢失，并有助于 HFD 大鼠的骨形成。
