短期饮食镁缺乏下调大鼠骨形成相关基因的表达。

Short-term dietary magnesium deficiency downregulates the expression of bone formation-related genes in rats.

出版信息

Magnes Res. 2023 Sep 1;36(3):23-29. doi: 10.1684/mrh.2023.0518.

DOI:10.1684/mrh.2023.0518

Abstract

Dietary magnesium deficiency increases osteoclastic bone resorption and decreases osteoblastic bone formation. Increased bone resorption due to dietary magnesium deficiency can be explained by increased expression of the receptor activator of nuclear factor kB ligand. However, the detailed mechanisms underlying decreased bone formation remain unclear. Thus, in the present study, to determine the mechanism underlying decreased bone formation induced by dietary magnesium deficiency, we investigated the effects of short-term dietary magnesium deficiency on the mRNA expression of genes related to bone formation in rats. Male Wistar rats were fed a control or magnesium-deficient diet for eight days. The mRNA expression level of Runx2, Sp7, Bglap, Alpl, Col1a1, Igf1, and Bmp2 in the femur was significantly lower in magnesium-deficient rats than in control rats. These results suggest that short-term dietary magnesium deficiency decreases the gene expression of insulin-like growth factor-1 and bone morphogenetic protein 2, which, in turn, decreases osteoblastic bone formation through the downregulation of osteoblastogenesis-related gene expression.

摘要

饮食性镁缺乏会增加破骨细胞的骨吸收，减少成骨细胞的骨形成。饮食性镁缺乏引起的骨吸收增加可以通过核因子κB 配体受体激活剂表达增加来解释。然而，骨形成减少的详细机制仍不清楚。因此，在本研究中，为了确定饮食性镁缺乏诱导的骨形成减少的机制，我们研究了短期饮食性镁缺乏对大鼠骨形成相关基因 mRNA 表达的影响。雄性 Wistar 大鼠分别用对照或镁缺乏饮食喂养 8 天。镁缺乏大鼠股骨中 Runx2、Sp7、Bglap、Alpl、Col1a1、Igf1 和 Bmp2 的 mRNA 表达水平明显低于对照组。这些结果表明，短期饮食性镁缺乏会降低胰岛素样生长因子-1 和骨形态发生蛋白 2 的基因表达，从而通过下调成骨细胞生成相关基因的表达，减少成骨细胞的骨形成。