Matsuoka Shunichiro, Hara Daisuke, Nakamura Daisuke, Kumeda Hirotaka, Miura Kentaro, Iwaya Mai, Eguchi Takashi, Hamanaka Kazutoshi, Uehara Takeshi, Shimizu Kimihiro
Division of General Thoracic Surgery, Department of Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Department of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Gen Thorac Cardiovasc Surg. 2024 Oct 28. doi: 10.1007/s11748-024-02098-8.
We investigated and compared the long-term (6-month) histologic changes in a rat model of sublobar resection created using electrocautery or stapler techniques.
Nine-week-old male rats were anesthetized and intubated; thoracotomy with sublobar resection was performed in the right middle lobe using electrocautery or stapler techniques. Histological examination was performed at 2, 4, 8, 12, and 24 weeks post-surgery to assess long-term effects on lung tissue repair and morphologic changes. Lung expansion and alveolar epithelial cell proliferation were evaluated by measuring the mean linear intercept and counting the number of alveolar type I and II cells.
The electrocautery group showed signs of lung self-repair at the resected area over time, with inflammatory cell infiltration followed by growth of vessels and bronchioles. Mesothelial cells covered the resected area by 2 weeks; elastic fibers gradually connected from both sides by 24 weeks. Lung expansion, measured by mean linear intercept, was initially small below the electrocautery resection area at 2 weeks but recovered from 4 to 24 weeks. The stapler group showed persistently small mean linear intercept over time. In the electrocautery group, the number of alveolar type II cells was higher just below the resection than in other areas from 2 to 24 weeks, followed by alveolar type I cells (4 to 24 weeks). The stapler group showed a transient alveolar type II cell increase at 2 weeks.
Compared to the stapler technique, electrocautery may provide advantages for postoperative lung repair by promoting lung expansion and alveolar epithelial cell proliferation.
我们研究并比较了使用电灼术或吻合器技术建立的大鼠肺叶下切除模型的长期（6个月）组织学变化。
将9周龄雄性大鼠麻醉并插管；使用电灼术或吻合器技术在右中叶进行开胸肺叶下切除术。在术后2、4、8、12和24周进行组织学检查，以评估对肺组织修复和形态学变化的长期影响。通过测量平均线性截距并计数I型和II型肺泡细胞数量来评估肺扩张和肺泡上皮细胞增殖。
电灼术组随着时间推移在切除区域显示出肺自我修复的迹象，先是炎性细胞浸润，随后是血管和细支气管生长。间皮细胞在2周时覆盖切除区域；弹性纤维在24周时从两侧逐渐连接。以平均线性截距衡量的肺扩张在术后2周时电灼术切除区域下方最初较小，但在4至24周恢复。吻合器组随着时间推移平均线性截距持续较小。在电灼术组，从2至24周，切除区域下方的II型肺泡细胞数量高于其他区域，随后是I型肺泡细胞（4至24周）。吻合器组在2周时出现II型肺泡细胞短暂增加。
与吻合器技术相比，电灼术可能通过促进肺扩张和肺泡上皮细胞增殖为术后肺修复提供优势。
