Medical Information College, Chongqing Medical University, Chongqing 400016, People's Republic of China.
Banan Hospital Affiliated to Chongqing Medical University, Chongqing 401320, People's Republic of China.
Biomed Mater. 2024 Nov 14;20(1). doi: 10.1088/1748-605X/ad8c09.
As the incidence of chronic wounds increases, the requirements for wound dressings are rising. The specific aim of this study is to propose a novel gallic acid (GA) functionalized silk fibroin (SF) and gelatin (Gel) composite wound dressing in which GA is used as an antibacterial and wound healing substance. Via electrospinning, SF, Gel, and GA mixed solutions could be conveniently fabricated into a composite nanofiber mat (SF-Gel-GA), consisting of uniform fibers with an average diameter around 134.57 ± 84 nm. The internal mesh structure of SF-Gel-GA provides sufficient drug loading capacity, proper moisture permeability, and proper degradation rate. SF-Gel-GA presents excellent biocompatibility. NIH-3T3 fibroblast cells could adhere and spread stably on the SF-Gel-GA surface with slightly promoted proliferation. In the presence of SF-Gel-GA, the growth of both Gram-positive and Gram-negative bacteria, includingand, is significantly inhibited in both plate and suspension cultures. A cutaneous excisional mouse wound model proves the efficient ability of SF-Gel-GA to promote wound healing. Compared with pure SF dressing and commercial Tegaderm Hydrocolloiddressing, the wound closure rate with SF-Gel-GA treatment is significantly improved. The histological assessments further demonstrate SF-Gel-GA could facilitate collagen deposition, neovascularization, and epithelialization at wound sites to promote wound healing. In conclusion, a novel SF-Gel-GA composite wound dressing with efficient wound healing activities have been developed for chronic wound treatment with broad healing potential.
随着慢性伤口发病率的增加，对伤口敷料的需求也在不断上升。本研究的具体目的是提出一种新型的没食子酸（GA）功能化丝素（SF）和明胶（Gel）复合伤口敷料，其中 GA 用作抗菌和伤口愈合物质。通过静电纺丝，可以方便地将 SF、Gel 和 GA 混合溶液制成复合纳米纤维垫（SF-Gel-GA），由平均直径约为 134.57 ± 84nm 的均匀纤维组成。SF-Gel-GA 的内部网状结构提供了足够的药物负载能力、适当的透湿性和适当的降解率。SF-Gel-GA 具有良好的生物相容性。NIH-3T3 成纤维细胞可以稳定地附着和铺展在 SF-Gel-GA 表面，并且增殖略有促进。在 SF-Gel-GA 的存在下，革兰氏阳性和革兰氏阴性菌（包括 和 ）的生长在平板和悬浮培养中均受到显著抑制。皮肤切除小鼠伤口模型证明了 SF-Gel-GA 促进伤口愈合的有效能力。与纯 SF 敷料和商业 Tegaderm Hydrocolloid 敷料相比，SF-Gel-GA 处理的伤口闭合率显著提高。组织学评估进一步表明，SF-Gel-GA 可以促进伤口部位的胶原沉积、新血管生成和上皮化，从而促进伤口愈合。总之，开发了一种具有高效伤口愈合活性的新型 SF-Gel-GA 复合伤口敷料，用于慢性伤口治疗，具有广泛的愈合潜力。
