Department of Natural Products, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, Dammam 31441, Kingdom of Saudi Arabia.
Ultrason Sonochem. 2024 Dec;111:107128. doi: 10.1016/j.ultsonch.2024.107128. Epub 2024 Oct 26.
Dolomiaea costus, commonly known as Indian costus, is a medicinal plant from the Asteraceae family. The root and powder of costus have been widely used to treat various health conditions. The primary bioactive compounds in this plant are sesquiterpene lactones, particularly costunolide and dehydrocostus lactone. This study aimed to establish a rapid, environmentally friendly, and cost-effective method for the high-throughput extraction and quantification of sesquiterpene lactones in Indian costus. Ultrasonic bath (UB) and UPLC/MS-MS were employed to extract and analyse 49 Indian costus samples. Aqueous ethanol was identified as the most effective solvent system for extracting and analysing sesquiterpene lactones. The extraction efficiency of the ultrasonic bath was comparable to that of the ultrasonic homogeniser while shaking showed the lowest efficiency. The environmentally friendly UPLC/MS-MS analysis revealed mean concentrations (±SD; μg/100 μg) of 1.00 (±0.39) for costunolide and 0.70 (±0.25) for dehydrocostus lactone. An inverse correlation was observed between sesquiterpene lactone content and sample colour. Most samples contained costunolide levels above the minimum limit (0.6 %) specified by the Chinese monograph, but only a few met the 1.8 % threshold for total sesquiterpene lactones. Given the importance of bioactive sesquiterpene lactones for medicinal efficacy, insufficient levels may result in diminished therapeutic value. Therefore, standardising Indian costus products is crucial to ensure quality and appropriate dosing. This study contributes to the standardisation of Indian costus, a vital step towards ensuring the efficacy and safety of herbal products.
多裂骆驼蓬，俗称印度骆驼蓬，是菊科药用植物。其根和粉末被广泛用于治疗各种健康状况。该植物的主要生物活性化合物为倍半萜内酯，特别是寇醇内酯和去氢骆驼蓬碱。本研究旨在建立一种快速、环保且具有成本效益的高通量提取和定量分析印度骆驼蓬中倍半萜内酯的方法。超声浴（UB）和 UPLC/MS-MS 被用于提取和分析 49 个印度骆驼蓬样本。水乙醇被确定为提取和分析倍半萜内酯最有效的溶剂系统。超声浴的提取效率与超声匀浆器相当，而摇床的提取效率最低。环保的 UPLC/MS-MS 分析显示，寇醇内酯的平均浓度（±SD；μg/100μg）为 1.00（±0.39），去氢骆驼蓬碱的平均浓度为 0.70（±0.25）。倍半萜内酯含量与样品颜色呈负相关。大多数样品的寇醇内酯含量高于中国专论规定的最低限度（0.6%），但只有少数样品的总倍半萜内酯含量达到 1.8%的阈值。鉴于生物活性倍半萜内酯对药用功效的重要性，含量不足可能会降低治疗价值。因此，标准化印度骆驼蓬产品对于确保质量和适当剂量至关重要。本研究为印度骆驼蓬的标准化做出了贡献，是确保草药产品功效和安全性的重要一步。
