Kour Sumeet, Singh Seema, Wani Tareq A, Kaloo Zahoor A
Plant Tissue Culture Lab, Department of Botany, University of Kashmir, Hazratbal, Srinagar, Jammu and Kashmir 190006 India.
Physiol Mol Biol Plants. 2023 Jan;29(1):87-91. doi: 10.1007/s12298-022-01270-9. Epub 2022 Dec 23.
(Asteraceae) commonly known as kuth, is an important medicinal plant with a rich repository of medicinally valuable compounds. During the present study, pharmacologically important sesquiterpene lactones namely costunolide, dehydrocostus lactone, betulinic acid and syringin were isolated from different plant extracts. Furthermore, the elicitation effect of jasmonic acid (JA) and different light regiments on the accumulation of secondary metabolites (costunolide and dehydrocostus lactone) was evaluated using HPLC. There was an increase in amount of costunolide and dehydrocostus lactone compared to control after 96 h of treatment with JA and continuous light. The amount of costunolide after 96 h was maximum 6.47 mg/g DW in response to JA as compared to control which was found to be 1.7 mg/g DW. Similarly, the concentration of dehydrocostus lactone after 96 h showed maximum accumulation of compound 4.7 mg/g DW in response to continuous light. The in vitro response in MS medium augmented with BAP (4 mg/l) produces friable and creamish coloured callus, however, number of days increased from 10 to 22 days with 70% culture response. Also, strain LBA9402 was found to be most effective strain for the establishment of hairy root cultures among all the strains used. The genomic DNA was used as template in PCR to amplify gene which confirmed the efficient transformation of the roots. Additionally, total metabolite content of in vitro raised hairy roots of was significantly higher than the field grown plants. The production of secondary metabolites through elicitation and hairy roots can serve as a potential tool for the conservation action programme in
The online version contains supplementary material available at 10.1007/s12298-022-01270-9.
菊科植物（通常称为库斯）是一种重要的药用植物，含有丰富的具有药用价值的化合物。在本研究中，从不同植物提取物中分离出了具有药理学重要性的倍半萜内酯，即木香烃内酯、去氢木香内酯、桦木酸和丁香苷。此外，使用高效液相色谱法评估了茉莉酸（JA）和不同光照条件对次生代谢产物（木香烃内酯和去氢木香内酯）积累的诱导作用。与对照相比，在用JA处理96小时并持续光照后，木香烃内酯和去氢木香内酯的含量有所增加。与对照（对照含量为1.7mg/g干重）相比，用JA处理96小时后，木香烃内酯的含量最高可达6.47mg/g干重。同样，持续光照96小时后，去氢木香内酯的浓度显示该化合物的最大积累量为4.7mg/g干重。在添加了BAP（4mg/l）的MS培养基中的体外反应产生了易碎的奶油色愈伤组织，然而，培养天数从10天增加到22天，培养响应率为70%。此外，在所有使用的菌株中，发现菌株LBA9402是建立毛状根培养最有效的菌株。基因组DNA用作PCR模板来扩增基因，这证实了根的有效转化。此外，体外培养的毛状根的总代谢物含量明显高于田间种植的植物。通过诱导和毛状根产生次生代谢产物可作为保护行动计划的潜在工具
在线版本包含可在10.1007/s12298-022-01270-9获取的补充材料。
