Evans T M, Seidler R J, LeChevallier M W
Appl Environ Microbiol. 1981 May;41(5):1144-51. doi: 10.1128/aem.41.5.1144-1151.1981.
Verification of membrane filter total coliform colonies was compared in lauryl tryptose broth, and m-LAC broth primary media and brilliant green-lactose-bile broth and EC broth secondary media. Verification in m-LAC broth yielded the greatest number of aerogenic isolates for both untreated surface water and drinking water samples. Verification in brilliant green-lactose-bile broth increased the number of false-negative reactions. At least 90% of the isolates aerogenic in primary verification media and anaerogenic in brilliant green-lactose-bile broth were representative of the coliform genera. The addition of a resuscitation step in the membrane filter technique did not yield greater numbers of verified coliforms per sample. Verification of both typical and atypical colonies in m-LAC broth resulted in a 10-fold increase in coliform numbers from untreated surface water. With drinking water, verification of both colony types resulted in an increase from less than 1 coliform per 100 ml to greater than 1/100 ml. A single-step verification in m-LAC broth is proposed as a more rapid and sensitive coliform verification procedure than the standard technique.
在月桂基胰蛋白胨肉汤、m-LAC肉汤基础培养基、亮绿乳糖胆盐肉汤和EC肉汤二级培养基中，对膜滤法检测的总大肠菌群菌落进行了验证比较。对于未经处理的地表水和饮用水样本，在m-LAC肉汤中进行验证产生的产气分离株数量最多。在亮绿乳糖胆盐肉汤中进行验证增加了假阴性反应的数量。在基础验证培养基中产气而在亮绿乳糖胆盐肉汤中不产气的分离株中，至少90%属于大肠菌群属。在膜滤法技术中增加复苏步骤并没有使每个样本中经验证的大肠菌群数量增加。在m-LAC肉汤中对典型菌落和非典型菌落进行验证，使未经处理的地表水中大肠菌群数量增加了10倍。对于饮用水，对两种菌落类型进行验证后，每100毫升中的大肠菌群数量从少于1个增加到大于1个/100毫升。建议在m-LAC肉汤中进行单步验证，作为一种比标准技术更快速、灵敏的大肠菌群验证方法。
