Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), Department of Laboratory Medicine, Chongqing Medical University, Chongqing, China.
Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Nat Commun. 2024 Oct 28;15(1):9304. doi: 10.1038/s41467-024-53693-5.
Invasive fungal infections (IFIs) pose a significant threat to immunocompromised individuals, leading to considerable morbidity and mortality. Prompt and accurate diagnosis is essential for effective treatment. Here we develop a rapid molecular diagnostic method that involves three steps: fungal enrichment using affinity-magnetic separation (AMS), genomic DNA extraction with silicon hydroxyl magnetic beads, and detection through a one-pot system. This method, optimized to detect 30 CFU/mL of C. albicans in blood and bronchoalveolar lavage (BAL) samples within 2.5 h, is approximately 100 times more sensitive than microscopy-based staining. Initial validation using clinical samples showed 93.93% sensitivity, 100% specificity, and high predictive values, while simulated tests demonstrated 95% sensitivity and 100% specificity. This cost-effective, highly sensitive technique offers potential for use in resource-limited clinical settings and can be easily adapted to differentiate between fungal species and detect drug resistance.
侵袭性真菌感染（IFIs）对免疫功能低下的个体构成重大威胁，导致相当高的发病率和死亡率。及时准确的诊断对于有效治疗至关重要。在这里，我们开发了一种快速的分子诊断方法，该方法涉及三个步骤：使用亲和磁分离（AMS）进行真菌富集、使用硅羟基磁珠提取基因组 DNA，以及通过一锅系统进行检测。该方法针对血液和支气管肺泡灌洗液（BAL）样本中 30 CFU/mL 的 C. albicans 进行了优化，可在 2.5 小时内检测到，其灵敏度比基于显微镜的染色法高约 100 倍。使用临床样本进行的初步验证显示，该方法的灵敏度为 93.93%，特异性为 100%，预测值高，而模拟测试显示灵敏度为 95%，特异性为 100%。这种具有成本效益、高灵敏度的技术具有在资源有限的临床环境中使用的潜力，并且可以很容易地适应于区分真菌物种和检测耐药性。
