Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China.
Laboratory of Clinical Pharmacy, Department of Pharmacy, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China.
Nano Lett. 2024 Nov 13;24(45):14337-14345. doi: 10.1021/acs.nanolett.4c04026. Epub 2024 Oct 29.
Nanomaterials with peroxidase-like activity and photothermal conversion efficiency have garnered significant attention for their ability to generate cytotoxic hydroxyl radicals and provide synergistic therapeutic effects. Selecting nanozymes with suitable properties and carriers is crucial for maximizing efficacy. While the mucin family is known for its mucoadhesive, glycosylated structures that enhance drug bioavailability and targeting, its potential in nanozymes remains underexplored. Here, we utilize mucin-2 to facilitate osmium nanoclusters (Os@Mucin), creating protein-corona-like nanozymes. This configuration bestows Os@Mucin with excellent peroxidase-like activity (769 U/mg) and photothermal conversion efficiency (22.83%, 808 nm). Mucin-2 promotes Os uptake by cells, allowing Os@Mucin to exhibit tumor environment-responsive peroxidase-like activity, further enhanced under photothermal conditions for targeted cytotoxicity and synergistic effects. experiments demonstrate that this integration effectively treats triple-negative breast cancer. This study innovatively highlights the potential of the mucin family and underscores the promising role of Os nanozymes in tumor therapy.
具有过氧化物酶样活性和光热转换效率的纳米材料因其能够产生细胞毒性羟自由基并提供协同治疗效果而受到广泛关注。选择具有合适性质和载体的纳米酶对于最大限度地提高疗效至关重要。尽管粘蛋白家族以其具有粘弹性、糖基化结构而闻名，可提高药物的生物利用度和靶向性，但它们在纳米酶中的应用潜力尚未得到充分探索。在这里，我们利用粘蛋白-2 来促进锇纳米团簇（Os@Mucin）的形成，从而构建出具有蛋白冠状结构的纳米酶。这种结构使 Os@Mucin 具有优异的过氧化物酶样活性（769 U/mg）和光热转换效率（22.83%，808nm）。粘蛋白-2 促进了细胞对 Os 的摄取，使 Os@Mucin 能够表现出肿瘤微环境响应性的过氧化物酶样活性，在光热条件下进一步增强，以实现靶向细胞毒性和协同作用。实验表明，这种整合有效地治疗了三阴性乳腺癌。本研究创新性地强调了粘蛋白家族的潜力，并突出了 Os 纳米酶在肿瘤治疗中的应用前景。
