The Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, China.
Basic Medical School, Zhejiang Chinese Medical University, Hangzhou, China.
Medicine (Baltimore). 2024 Oct 25;103(43):e40180. doi: 10.1097/MD.0000000000040180.
To investigate the potential link between gut microbiota and functional dyspepsia (FD). Genome-wide association studies (GWAS) of gut microbiota and FD were used in Mendelian randomization (MR) research. Using the GWAS of 18,340 people, instrumental variables related to gut microbiota as an exposure factor were identified. In a GWAS investigation, 189,695 control individuals and 4376 FD patients were included as outcome variables. The primary analysis technique was inverse variance weighted analysis. The reliability of MR analysis results is tested using sensitivity analysis. Two-sample Mendelian randomization analysis revealed the presence of 7 gut microbiota associated to FD. In the inverse variance weighted analysis method, Order Erysipelotrichales (odds ratio (OR): 1.301; 95% confidence interval (CI): 1.016, 1.665; P = .037), Family Erysipelotrichales (OR: 1.301; 95% CI: 1.016, 1.665; P = .037), Genus Haemophilus (OR: 1.236; 95% CI 1.059, 1.442; P = .007), Genus Ruminiclostridium 9 (OR: 1.422; 95% CI: 1.078, 1.877; P = .013), Genus Lachnospiraceae NK4A 136 group (OR: 1.297; 95% CI: 1.059, 1.589; P = .012) was positively associated with FD. Class Gammaproteobacteria (OR: 0.705; 95% CI: 0.522, 0.952; P = .022) and Genus Erysipelatoclostridium (OR: 0.747; 95% CI: 0.628, 0.888; P = .001) were found to be inversely related to FD. There was no evidence of pleiotropy or heterogeneity in the sensitivity analysis. Our research provides evidence for a possible link between FD and a number of gut microbiota. The role that gut microbiota plays in the development of FD requires more investigation.
为了研究肠道微生物群与功能性消化不良（FD）之间的潜在联系。采用孟德尔随机化（MR）研究对肠道微生物群和 FD 的全基因组关联研究（GWAS）进行了分析。使用 18340 人的 GWAS，确定了与肠道微生物群相关的工具变量作为暴露因素。在 GWAS 研究中，将 189695 名对照个体和 4376 名 FD 患者作为结果变量。主要分析技术是逆方差加权分析。采用敏感性分析检验 MR 分析结果的可靠性。两样本 Mendelian 随机化分析显示，有 7 种肠道微生物群与 FD 相关。在逆方差加权分析方法中，Order Erysipelotrichales（比值比（OR）：1.301；95%置信区间（CI）：1.016，1.665；P=0.037）、Family Erysipelotrichales（OR：1.301；95%CI：1.016，1.665；P=0.037）、属嗜血杆菌（OR：1.236；95%CI 1.059，1.442；P=0.007）、属 Ruminiclostridium 9（OR：1.422；95%CI：1.078，1.877；P=0.013）、属 Lachnospiraceae NK4A 136 组（OR：1.297；95%CI：1.059，1.589；P=0.012）与 FD 呈正相关。类γ变形菌（OR：0.705；95%CI：0.522，0.952；P=0.022）和属 Erysipelatoclostridium（OR：0.747；95%CI：0.628，0.888；P=0.001）与 FD 呈负相关。敏感性分析未发现偏倚或异质性。我们的研究为 FD 与多种肠道微生物群之间可能存在联系提供了证据。肠道微生物群在 FD 发展中的作用需要进一步研究。
