Cervantes Jorge, Michael Majd, Hong Bo-Young, Springer Aden, Guo Hua, Mendoza Burgandy, Zeng Mingtao, Sundin Olof, McCallum Richard
Texas Tech University Health Sciences Center El Paso Paul L Foster School of Medicine, El Paso, Texas, USA.
The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
J Investig Med. 2020 Dec 17. doi: 10.1136/jim-2020-001642.
Disease-associated alterations of the intestinal microbiota composition, known as dysbiosis, have been well described in several functional gastrointestinal (GI) disorders. Several studies have described alterations in the gastric microbiota in functional dyspepsia, but very few have looked at the duodenum.Here, we explored the upper GI tract microbiota of inpatients with upper GI dyspeptic symptoms, and compared them to achalasia controls, as there is no indication for an esophagogastroduodenoscopy in healthy individuals.We found differences in the microbiota composition at the three sites evaluated (ie, saliva, stomach and duodenum). Changes observed in patients with dyspepsia included an increase in in saliva, an oral shift in the composition of the gastric microbiota, and to some degree in the duodenum as well, where an important abundance of anaerobes was observed. Metabolic function prediction identified greater anaerobic metabolism in the stomach microbial community of patients with dyspepsia. Proton pump inhibitor use was not associated with any particular genus. Co-abundance analysis revealed as the main hub in the duodenum, a genus that significantly correlated with the relative abundance of , and We conclude that patients with upper GI symptoms consistent with dyspepsia have alterations in the microbiota of saliva, the stomach, and duodenum, which could contribute to symptoms of functional GI disorders.
肠道微生物群组成的疾病相关改变，即生态失调，已在几种功能性胃肠（GI）疾病中得到充分描述。多项研究描述了功能性消化不良患者胃微生物群的改变，但很少有研究关注十二指肠。在此，我们探索了有上消化道消化不良症状的住院患者的上消化道微生物群，并将其与贲门失弛缓症对照组进行比较，因为健康个体没有进行食管胃十二指肠镜检查的指征。我们发现在评估的三个部位（即唾液、胃和十二指肠）微生物群组成存在差异。消化不良患者观察到的变化包括唾液中[未提及具体物质]增加、胃微生物群组成的口腔转移以及十二指肠在一定程度上的转移，在十二指肠观察到大量厌氧菌。代谢功能预测表明消化不良患者胃微生物群落中厌氧代谢更强。质子泵抑制剂的使用与任何特定菌属无关。共丰度分析显示[未提及具体菌属]是十二指肠中的主要枢纽菌属，该菌属与[未提及具体菌属]的相对丰度显著相关，并且我们得出结论，有与消化不良相符的上消化道症状的患者在唾液、胃和十二指肠的微生物群存在改变，这可能导致功能性胃肠疾病的症状。
