Premarathna Amal D, Ahmed Tamer A E, Rjabovs Vitalijs, Critchley Alan T, Hincke Maxwell T, Tuvikene Rando
School of Natural Sciences and Health, Tallinn University, Narva mnt 29, 10120 Tallinn, Estonia.
Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ontario, K1H 8M5, Canada; School of Nutrition Sciences, Faculty of Health Sciences, University of Ottawa, Ontario, K1H 8M5, Canada.
Int J Biol Macromol. 2024 Dec;282(Pt 4):136858. doi: 10.1016/j.ijbiomac.2024.136858. Epub 2024 Oct 28.
This research work explores the physicochemical characteristics and biological functions of polysaccharides extracted from four selected green seaweed species: Ulva lactuca (UL), Halimeda opuntia (HO), Caulerpa racemosa (CR), and Chaetomorpha antennina (CA). The extracts were investigated for cell-based bio-activities (i.e., cytotoxicity, cell proliferation and migration) using three cell lines (HDF, HaCaT, RAW264.7) reflecting cell models involved in wound healing, as well as anticoagulant activity. Ulvans from UL significantly increased HaCaT (at 0.06 μg/μL) and HDF (at 0.5 μg/μL) cell proliferation. In addition, extracts from CA showed the highest cell migration ability using HDF and HaCaT cells. UL (all fractions), HO-2A, CR-1B, CA-1A and CA-2B fractions improved phagocytosis. Furthermore, RAW264.7 cells treated with fraction CA-1A produced significantly more intracellular NO (pro-inflammatory) within 24 h compared to control (LPs). Green seaweed extracts CA-2A and UL-1A resulted in lower expression of the pro-inflammatory cytokine TNF-α in skin cells (HDF, HaCaT). Caulerpa cold-extracted polysaccharides possessed higher anticoagulant properties. The Ulva (1,4-linked α-l-rhamnose, β-d-glucuronic acid, l-iduronic acid) and CA extracts are promising sources of bioactive therapeutic agents. Our data provide useful insights into the possible biomedical benefits of selected polysaccharides mixtures (i.e., ulvan, sulfated or/and pyruvylated β-d-galactans, sulfated xyloarabinogalactan) for food, pharmaceutical, and biotechnological applications.
本研究工作探索了从四种选定的绿色海藻物种中提取的多糖的物理化学特性和生物学功能，这四种海藻分别是：石莼（UL）、仙人掌藻（HO）、总状蕨藻（CR）和鹿角沙菜（CA）。使用反映参与伤口愈合的细胞模型的三种细胞系（人皮肤成纤维细胞HDF、人永生化表皮细胞HaCaT、小鼠单核巨噬细胞RAW264.7）对提取物进行基于细胞的生物活性（即细胞毒性、细胞增殖和迁移）以及抗凝血活性研究。来自石莼的石莼聚糖在浓度为0.06μg/μL时显著提高了HaCaT细胞的增殖，在浓度为0.5μg/μL时显著提高了HDF细胞的增殖。此外，鹿角沙菜的提取物在使用HDF和HaCaT细胞时表现出最高的细胞迁移能力。石莼（所有组分）、仙人掌藻-2A、总状蕨藻-1B、鹿角沙菜-1A和鹿角沙菜-2B组分改善了吞噬作用。此外，与对照组（脂多糖LPs）相比，用鹿角沙菜-1A组分处理的RAW264.7细胞在24小时内产生了显著更多的细胞内一氧化氮（促炎因子）。绿色海藻提取物鹿角沙菜-2A和石莼-1A导致皮肤细胞（HDF、HaCaT）中促炎细胞因子肿瘤坏死因子-α的表达降低。总状蕨藻冷提取的多糖具有更高的抗凝血特性。石莼（1,4-连接的α-L-鼠李糖、β-D-葡萄糖醛酸、L-艾杜糖醛酸）和鹿角沙菜提取物有望成为生物活性治疗剂的来源。我们的数据为选定的多糖混合物（即石莼聚糖、硫酸化或/和丙酮酸化的β-D-半乳聚糖、硫酸化木糖阿拉伯半乳聚糖）在食品、制药和生物技术应用中的潜在生物医学益处提供了有用的见解。
