Yan Zuhua, Jin Hui, Yang Xiaoyan, Min Deng, Xu Xinxin, Hua Cuiping, Qin Bo
Research Center for Natural Medicine and Chemical Metrology and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, Gansu, 730000, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Folia Microbiol (Praha). 2024 Oct 29. doi: 10.1007/s12223-024-01210-y.
The growth and accumulation of active ingredients of Angelica sinensis were affected by rhizosphere soil microbial communities and soil environmental factors. However, the correlationship between growth and active ingredients and soil biotic and abiotic factors is still unclear. This study explored rhizosphere soil microbial community structures, soil physicochemical properties, enzyme activities, and their effects on the growth and active ingredient contents of A. sinensis in three principal cropping areas. Results indicated that the growth indices, ligustilide, ferulic acid contents, and soil environmental factors varied in cropping areas. Pearson correlation analysis revealed that the growth of A. sinensis was affected by organic matter, total nitrogen, total phosphorus, and available phosphorus; ferulic acid and ligustilide accumulation were related to soil catalase and alkaline phosphatase activities, respectively. Illumina MiSeq sequencing showed that the genera Mortierella and Conocybe were the dominant fungal communities, and Sphingomonas, Pseudomonas, Bryobacter, and Lysobacter were the main bacterial communities associated with the rhizosphere soil. Kruskal-Wallis one-way ANOVA and Spearman correlation conjoint analysis demonstrated a significant positive correlation (p < 0.001) among the composition of the rhizosphere microbial communities at all three sampling sites. The growth and active ingredient accumulation of A. sinensis not only was significantly susceptible to the bacterial communities of Sphingomonas, Epicoccum, Marivita, Muribaculum, and Gemmatimonas but also were significantly influenced by the fungal communities of Inocybe, Septoria, Tetracladium, and Mortierella (p < 0.05). Our findings provide a scientific basis for understanding the relationship between the growth and active ingredients in A. sinensis and their corresponding rhizosphere soil microbial communities, soil physicochemical properties, and enzyme activities.
当归活性成分的生长和积累受根际土壤微生物群落和土壤环境因子的影响。然而，其生长、活性成分与土壤生物和非生物因子之间的相关性仍不清楚。本研究探讨了三个主要种植区当归根际土壤微生物群落结构、土壤理化性质、酶活性及其对当归生长和活性成分含量的影响。结果表明，不同种植区的生长指标、藁本内酯、阿魏酸含量和土壤环境因子存在差异。Pearson相关性分析表明，当归的生长受有机质、全氮、全磷和有效磷的影响；阿魏酸和藁本内酯的积累分别与土壤过氧化氢酶和碱性磷酸酶活性有关。Illumina MiSeq测序表明，被孢霉属和小伞属是主要的真菌群落，鞘氨醇单胞菌属、假单胞菌属、Bryobacter属和溶杆菌属是与根际土壤相关的主要细菌群落。Kruskal-Wallis单因素方差分析和Spearman相关性联合分析表明，所有三个采样点的根际微生物群落组成之间存在显著正相关（p < 0.001）。当归的生长和活性成分积累不仅对鞘氨醇单胞菌属、附球菌属、Marivita属、Muribaculum属和芽单胞菌属的细菌群落敏感，而且还受到丝盖伞属、壳针孢属、四枝孢属和被孢霉属真菌群落的显著影响（p < 0.05）。我们的研究结果为理解当归生长、活性成分与其相应根际土壤微生物群落、土壤理化性质和酶活性之间的关系提供了科学依据。
