Department of Food Science and Technology, Quchan Branch, Islamic Azad University, Quchan, Iran.
Department of Food Processing, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran.
Sci Rep. 2024 Oct 29;14(1):25903. doi: 10.1038/s41598-024-77380-z.
Improving the quality of extracts derived from medicinal plants is a critical concern due to their extensive use across various industries. The Arvaneh plant (Hymenocrater platystegius Rech. F), a species native to the Khorasan province in Iran and belonging to the mint family, was the focus of this study. We optimized the extraction of Arvaneh plant using both microwave (MW) and pulsed electric field (PEF) techniques. The MW method was tested at different processing times (90, 180, and 270 s) and power levels (180, 540, and 900 W), while the PEF method was evaluated with varying electric field intensities (0.25, 3.25, and 6.25 kV/cm) and pulse numbers (10, 45, and 80) using a central composite design (CCD). The results revealed that extraction efficiency, total phenolic content, and total flavonoid content were significantly higher with the PEF method compared to the MW method (p < 0.05). Moreover, the PEF technique showed superior performance in preserving the antioxidant properties of the extract, as assessed by DPPH and FRAP methods. GC/MS analysis confirmed the presence of 27 bioactive compounds in the Arvaneh extract obtained through PEF-assisted extraction. In conclusion, the PEF method proved to be highly efficient for extracting bioactive compounds from the Arvaneh plant.
由于药用植物在各个行业中的广泛应用，提高其提取物的质量是一个至关重要的关注点。Arvaneh 植物（Hymenocrater platystegius Rech. F），一种原产于伊朗霍拉桑省的薄荷科植物，是本研究的重点。我们使用微波（MW）和脉冲电场（PEF）技术对 Arvaneh 植物的提取进行了优化。MW 方法在不同的处理时间（90、180 和 270 s）和功率水平（180、540 和 900 W）下进行了测试，而 PEF 方法则在不同的电场强度（0.25、3.25 和 6.25 kV/cm）和脉冲数（10、45 和 80）下进行了评估，采用中心复合设计（CCD）。结果表明，与 MW 方法相比，PEF 方法的提取效率、总酚含量和总黄酮含量显著更高（p < 0.05）。此外，PEF 技术在通过 DPPH 和 FRAP 方法评估的提取物抗氧化性能的保持方面表现出更好的性能。GC/MS 分析证实，通过 PEF 辅助提取获得的 Arvaneh 提取物中存在 27 种生物活性化合物。总之，PEF 方法被证明是从 Arvaneh 植物中提取生物活性化合物的高效方法。
