Shandong Second Medical University, Weifang, 261053, China.
Affiliated Hospital of Shandong Second Medical University, Weifang, 261031, China.
Sci Rep. 2024 Oct 29;14(1):25935. doi: 10.1038/s41598-024-77547-8.
Mosquito associated virus have always been a significant threat to global health. Metagenomics offers a straightforward and quantitative means to acquire the information of novel virus and has greatly enriched the content of mosquito associated virus databases. During an entomological surveillance for arthropod-borne viruses in China, we identified a previously unrecognized virus from mosquitoes, temporarily named Huajieling virus. In this study, a total of 3,960 mosquitoes were collected and then divided into 91 pools, according to location and species. QRT-PCR and nested PCR were performed to confirm the presence of Huajieling virus. Its genomic features and phylogenetic relationships were further analyzed. Our results showed that Huajieling virus was detected in 7 of the 91 mosquito pools and that the minimum infection rate (MIR) was 0.18% (7/3,960). One complete genome sequence and 2 viral partial sequences were obtained from the Huajieling virus-positive pools. Pairwise distances analysis indicated that these amplified sequences shared high nucleotide identity. Phylogenetic analysis demonstrated that Huajieling virus is most closely related to Wufeng shrew picorna-like virus 43, which belonging to Picornavirales. Further analyses indicated that Huajieling virus is a new member of unclassified Picornavirales, and is intermediate between the family Caliciviridae and Secoviridae in taxonomic status.
蚊媒相关病毒一直是全球健康的重大威胁。宏基因组学提供了一种直接且定量的方法来获取新病毒的信息，极大地丰富了蚊媒相关病毒数据库的内容。在中国进行的节肢动物传播病毒昆虫学监测中，我们从蚊子中鉴定出一种以前未被识别的病毒，暂时命名为花街岭病毒。在本研究中，共收集了 3960 只蚊子，然后根据地点和种类分为 91 个池。进行实时定量 PCR 和巢式 PCR 以确认花街岭病毒的存在。进一步分析了其基因组特征和系统发育关系。我们的结果显示，在 91 个蚊子池中检测到 7 个池中存在花街岭病毒，最低感染率（MIR）为 0.18%（7/3960）。从花街岭病毒阳性池中获得了 1 个完整基因组序列和 2 个病毒部分序列。序列比对分析表明，这些扩增序列具有高度的核苷酸同一性。系统发育分析表明，花街岭病毒与五峰猪尾鼠微小 RNA 样病毒 43 最为密切相关，属于微小 RNA 病毒目。进一步分析表明，花街岭病毒是未分类微小 RNA 病毒目新成员，在分类地位上介于杯状病毒科和长尾病毒科之间。
