Garson K, Kang C Y
Biochem Biophys Res Commun. 1986 Jan 29;134(2):716-22. doi: 10.1016/s0006-291x(86)80479-x.
The product of the v-rel oncogene has been identified as a 55,000 dalton protein using antiserum prepared against a synthetic polypeptide whose sequence was deduced from the DNA sequence of v-rel. This antiserum was used in a Western blot assay to identify the product of v-rel in chicken bone marrow cells transformed with reticuloendotheliosis virus, strain T (REV-T), and in Cos1 cells transfected with an expression vector containing the v-rel gene. Transient expression of v-rel under the transcriptional control of the SV40 late promoter in Cos1 cells leads to the synthesis of a rel specific protein with a similar molecular weight.
使用针对合成多肽制备的抗血清，已将v-rel癌基因的产物鉴定为一种55,000道尔顿的蛋白质，该合成多肽的序列是从v-rel的DNA序列推导出来的。这种抗血清用于蛋白质免疫印迹分析，以鉴定用T株网状内皮增生症病毒（REV-T）转化的鸡骨髓细胞以及用含有v-rel基因的表达载体转染的Cos1细胞中v-rel的产物。在Cos1细胞中，v-rel在SV40晚期启动子的转录控制下瞬时表达，导致合成一种分子量相似的rel特异性蛋白质。
