Ozawa T, Hayakawa M, Takamura T, Sugiyama S, Suzuki K, Iwata M, Taki F, Tomita T
Biochem Biophys Res Commun. 1986 Feb 13;134(3):1071-8. doi: 10.1016/0006-291x(86)90360-8.
In lung lavages of rat after pure oxygen breathing, a toxic linoleate peroxide, 9,10-epoxy-12-octadecenoate, and its isomer, 12,13-epoxy-9-octadecenoate were detected by HPLC analyses. The epoxide(s) was demonstrated to be biosynthesized by incubating linoleate with leukocytes collected from lung lavages, thus nominated to be leukotoxin. The chemical structures of leukotoxin and its isomer were determined by gas-chromatography/mass spectrometry and nuclear magnetic resonance measurements. Leukotoxin showed a potent uncoupling activity to rat liver mitochondrial respiration and a dose-dependent relaxation of rat stomach smooth muscle. These findings were discussed with 'oxygen toxicity' on the lung.
在纯氧呼吸后的大鼠肺灌洗物中，通过高效液相色谱分析检测到一种有毒的亚油酸过氧化物、9,10-环氧-12-十八碳烯酸及其异构体12,13-环氧-9-十八碳烯酸。通过将亚油酸与从肺灌洗物中收集的白细胞一起孵育，证明环氧化物是生物合成的，因此被命名为白细胞毒素。通过气相色谱/质谱和核磁共振测量确定了白细胞毒素及其异构体的化学结构。白细胞毒素对大鼠肝线粒体呼吸表现出强烈的解偶联活性，并对大鼠胃平滑肌有剂量依赖性舒张作用。结合肺的“氧中毒”对这些发现进行了讨论。
