Hayakawa M, Sugiyama S, Takamura T, Yokoo K, Iwata M, Suzuki K, Taki F, Takahashi S, Ozawa T
Biochem Biophys Res Commun. 1986 May 29;137(1):424-30. doi: 10.1016/0006-291x(86)91227-1.
An epoxy derivative of linoleate, 9,10-epoxy-12-octadecenoate, was demonstrated to be biosynthesized by neutrophils from various sources such as canine and human blood, and guinea-pig peritonea. It was nominated as leukotoxin from its 'toxic' activity onto mitochondrial respiration. From the reaction mixture of leukocytes with linoleate, an isomer of leukotoxin, 12,13-epoxy-9-octadecenoate, and a 'non-toxic' hydroxy derivative of linoleate, 9-hydroxy-12-octadecenoate, were detected. Such a cascade reaction of linoleate by leukocytes was discussed. Biosynthesis of leukotoxin by neutrophils was substantially enhanced by the presence of calcium ion and calcium-ionophore, A23187. Neutrophils contained leukotoxin, ca. 7 f moles/cell, which was extractable by 60% ethanol, but little of the isomer.
一种亚油酸的环氧衍生物，9,10-环氧-12-十八碳烯酸，已被证明可由来自各种来源（如犬类和人类血液以及豚鼠腹膜）的中性粒细胞进行生物合成。因其对线粒体呼吸具有“毒性”活性而被命名为白细胞毒素。从白细胞与亚油酸的反应混合物中，检测到了白细胞毒素的一种异构体，12,13-环氧-9-十八碳烯酸，以及亚油酸的一种“无毒”羟基衍生物，9-羟基-12-十八碳烯酸。讨论了白细胞对亚油酸的这种级联反应。钙离子和钙离子载体A23187的存在显著增强了中性粒细胞对白细胞毒素的生物合成。中性粒细胞含有白细胞毒素，约7飞摩尔/细胞，可被60%乙醇提取，但异构体含量很少。
