Li Zhiming, Xian Hongyi, Ren Xiaohu, Ye Rongyi, Zhong Yizhou, Huang Yuji, Liang Boxuan, Deng Yanhong, Dai Mingzhu, Guo Jie, Tang Shuqin, Pan Jialiang, Feng Yu, Bai Ruobing, Chen Xueping, Ichihara Sahoko, Ichihara Gaku, Chen Da, Yang Xingfen, Huang Zhenlie
NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Toxicology, School of Public Health, Southern Medical University, Guangzhou 510515, China.
Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of Health Toxicology, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
Environ Health (Wash). 2024 May 4;2(7):424-440. doi: 10.1021/envhealth.4c00045. eCollection 2024 Jul 19.
Triclosan (TCS) has garnered significant attention due to its widespread use and associated endocrine-disrupting effects. However, its impact on the neuroendocrine system and underlying mechanisms remain poorly understood. Here, we established correlations between TCS exposure and serum sex hormone levels in participants of the National Health and Nutrition Examination Survey (NHANES). Additionally, we investigated TCS's influence on the neuroendocrine system using adult zebrafish exposed to environmentally relevant concentrations of TCS (0.361-48.2 μg/L) for 21 days. Assessment of reproductive and neurotoxicity included histopathological examination and behavioral tests. Transcriptomics, proteomics analyses, and biochemical detection were employed to elucidate mechanisms underlying TCS-induced neuroendocrine disruption. Significant correlations were found between TCS exposure and estradiol, testosterone, and sex hormone-binding globulin levels in NHANES participants. In addition, TCS exposure inhibited ovary development and spermatogenesis in zebrafish. Transcriptomics and proteomics analysis revealed gender-specific key signaling and metabolism-related pathways implicated in TCS-induced reproductive toxicity. Moreover, TCS exposure induced nervous system impairment, as evidenced by histological changes and altered motor behavior, possibly associated with oxidative damage. Correlation analysis further highlighted the potential connection between endocrine system disruption and nervous system impairment following TCS exposure. Overall, this study provided evidence supporting TCS-induced endocrine disruption and offered insights into its underlying mechanisms.
三氯生（TCS）因其广泛使用及其相关的内分泌干扰作用而备受关注。然而，其对神经内分泌系统的影响及其潜在机制仍知之甚少。在此，我们在国家健康与营养检查调查（NHANES）的参与者中建立了三氯生暴露与血清性激素水平之间的相关性。此外，我们使用暴露于环境相关浓度三氯生（0.361 - 48.2 μg/L）21天的成年斑马鱼研究了三氯生对神经内分泌系统的影响。生殖和神经毒性评估包括组织病理学检查和行为测试。采用转录组学、蛋白质组学分析和生化检测来阐明三氯生诱导神经内分泌干扰的潜在机制。在NHANES参与者中发现三氯生暴露与雌二醇、睾酮和性激素结合球蛋白水平之间存在显著相关性。此外，三氯生暴露抑制了斑马鱼的卵巢发育和精子发生。转录组学和蛋白质组学分析揭示了三氯生诱导的生殖毒性中涉及的性别特异性关键信号传导和代谢相关途径。此外，三氯生暴露导致神经系统损伤，组织学变化和运动行为改变证明了这一点，这可能与氧化损伤有关。相关性分析进一步强调了三氯生暴露后内分泌系统破坏与神经系统损伤之间的潜在联系。总体而言，本研究提供了支持三氯生诱导内分泌干扰的证据，并深入了解了其潜在机制。
