Zhao Cheng, Wang Sijin, Liu Yuxi, Chu Peng, Han Bing, Ning Xianhui, Wang Tao, Yin Shaowu
College of Life Science, College of Marine Science and Engineering, Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, Nanjing Normal University, Nanjing, China; Co-Innovation Center for Marine Bio-Industry Technology, Lian Yungang, China.
College of Life Science, College of Marine Science and Engineering, Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, Nanjing Normal University, Nanjing, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Dec;48:101139. doi: 10.1016/j.cbd.2023.101139. Epub 2023 Sep 4.
Temperature is a critical factor that regulates the reproduction processes in teleost. However, the gonadal response mechanism to cold stress in fish remains largely unknown. In the present study, female zebrafish were exposed to different extents of low temperatures at 18 °C and 10 °C for 48 h. The ovarian histology was remarkably damaged after cold stress exposure. Integrated analysis of miRNA-mRNA was used to investigate the ovarian response to acute cold stress. A large number of mRNAs and miRNAs were altered by cold stress, which are involved in extensive biological processes. It is indicated that the signal transduction of MAPK and Calcium signaling pathway is highly engaged in zebrafish ovary to adapt to cold stress. The immune system was dysregulated by cold stress while the ovarian autophagy was activated. Remarkably increased gene number related to reproductive functions was identified in the cold stress at 10 °C compared to the control. The cold stress-induced dysregulated reproductive genes include star, hsd3b1, hsd17b1, inha, insl3, amh, nanos1 and foxl2. Combined with the dysregulated insulin, IGF and progesterone signaling, it is suggested that cold stress affects ovarian function in multiple aspects, including oocyte meiosis, folliculogenesis, final maturation and ovarian maintenance. On the other hand, the ovarian miRNA-mRNA regulatory network response to cold stress was also constructed. Overall, our result revealed the ovarian response to cold stress in zebrafish and provided insight into the fish adaptation mechanism to acute temperature change.
温度是调节硬骨鱼繁殖过程的关键因素。然而，鱼类性腺对冷应激的反应机制仍 largely 未知。在本研究中，将雌性斑马鱼在 18℃和 10℃下暴露于不同程度的低温 48 小时。冷应激暴露后卵巢组织学受到显著损伤。采用 miRNA-mRNA 综合分析来研究卵巢对急性冷应激的反应。大量的 mRNA 和 miRNA 因冷应激而改变，它们参与广泛的生物学过程。结果表明，MAPK 和钙信号通路的信号转导在斑马鱼卵巢中高度参与以适应冷应激。冷应激使免疫系统失调，同时激活了卵巢自噬。与对照组相比，在 10℃冷应激下鉴定出与生殖功能相关的基因数量显著增加。冷应激诱导的生殖基因失调包括 star、hsd3b1、hsd17b1、inha、insl3、amh、nanos1 和 foxl2。结合失调的胰岛素、IGF 和孕酮信号，提示冷应激在多个方面影响卵巢功能，包括卵母细胞减数分裂、卵泡发生、最终成熟和卵巢维持。另一方面，还构建了卵巢 miRNA-mRNA 对冷应激的调控网络。总体而言，我们的结果揭示了斑马鱼卵巢对冷应激的反应，并为鱼类对急性温度变化的适应机制提供了见解。
