Universidad Nacional de Colombia, Departamento de ciencias básicas y medicina oral, Facultad de Odontología, Bogotá, Colombia.
Pontificia Universidad Javeriana, Centro de investigaciones odontológicas, Facultad de Odontología, Bogotá, Colombia.
Braz Dent J. 2024 Oct 25;35:e245861. doi: 10.1590/0103-6440202405861. eCollection 2024.
This study quantified the production of the pro-resolving agent Resolvin E1 by peripheral blood mononuclear cells (PBMC) from 20 systemically healthy volunteers with and without periodontitis after stimulation with lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg). Ten periodontitis patients and 10 healthy volunteers (30-50 years old), matched by age and sex, were recruited. Peripheral blood mononuclear cells were isolated and stimulated in culture plates for 24 hours with Pg LPS. Resolvin E1 levels were measured in the supernatants by enzyme-linked immunosorbent assay. Significantly higher production of Resolvin E1 was observed in both groups when stimulated with LPS compared to baseline levels (p<0.001). A significant increase in Resolvin E1 was observed in the presence of Lipopolysaccharide in the patients with periodontitis compared to the healthy group (p=0.0019). Resolvin E1 levels may reflect a measure of resolution of inflammation that warrants further clinical investigation.
这项研究通过脂多糖（LPS）刺激来自 20 名健康志愿者（包括牙周炎患者和健康对照组）的外周血单个核细胞（PBMC），定量分析了促分解介质 Resolvin E1 的产生情况。我们招募了 10 名牙周炎患者和 10 名健康志愿者（年龄和性别相匹配，30-50 岁）。将外周血单个核细胞在培养板中与牙龈卟啉单胞菌 LPS 孵育 24 小时。通过酶联免疫吸附试验测量上清液中 Resolvin E1 的水平。与基线水平相比，LPS 刺激后两组的 Resolvin E1 产量均显著升高（p<0.001）。与健康对照组相比，牙周炎患者 LPS 存在时 Resolvin E1 显著增加（p=0.0019）。Resolvin E1 水平可能反映了炎症消退的程度，值得进一步的临床研究。
