Li Yige, Cheng Yingying, Ren Haoyi, Ji Tiantian, Zhao Zhengyi, Li Hongling, Hong Chenglin
School of Chemistry and Chemical Engineering/State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Shihezi University, Shihezi 832003, PR China.
School of Chemistry and Chemical Engineering/State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Shihezi University, Shihezi 832003, PR China.
Bioelectrochemistry. 2025 Feb;161:108843. doi: 10.1016/j.bioelechem.2024.108843. Epub 2024 Oct 26.
In this study, we developed a new system that using zinc-based metal-organic frameworks NH-Zn-PTC as the donor and ZiF-8@PDA as the acceptor to achieve highly sensitive detection of carcinoembryonic antigen (CEA), using the fundamentals of electrochemiluminescence resonance energy transfer (ECL-RET). Firstly, the aggregation-induced quenching effect (ACQ) was eliminated by the coordination of PTC in MOF and the ECL signal was improved. Secondly, the ECL signal was further amplified by using Au NPs and amino groups as co-reaction promoters to generate more SO. In addition, the introduction of ZiF-8@PDA as an acceptor and NH-Zn-PTC as a donor took advantage of the feature of partial overlap of the UV-vis absorption spectrum and ECL emission spectra between the two, thereby effectively initiating the ECL-RET behavior, which improved the detection sensitivity of the sensor. The prepared immunosensor showed good linearity in the concentration range of 10 to 80 ng/mL with a detection limit of 18.20 fg/mL. This makes it promising for clinical testing of tumor markers.
在本研究中，我们开发了一种新系统，该系统使用锌基金属有机框架NH-Zn-PTC作为供体，ZiF-8@PDA作为受体，利用电化学发光共振能量转移（ECL-RET）原理实现对癌胚抗原（CEA）的高灵敏度检测。首先，通过MOF中PTC的配位消除了聚集诱导猝灭效应（ACQ），并提高了ECL信号。其次，使用金纳米颗粒和氨基作为共反应促进剂以产生更多的SO，进一步放大了ECL信号。此外，引入ZiF-8@PDA作为受体和NH-Zn-PTC作为供体，利用了两者紫外-可见吸收光谱和ECL发射光谱部分重叠的特性，从而有效地引发了ECL-RET行为，提高了传感器的检测灵敏度。所制备的免疫传感器在10至80 ng/mL的浓度范围内显示出良好的线性，检测限为18.20 fg/mL。这使其在肿瘤标志物的临床检测中具有广阔前景。
