Xinjiang Key Laboratory of Energy Storage and Photoelectrocatalytic Materials, School of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, 830054, Xinjiang, P. R. China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, 410082, Hunan, P. R. China.
Mikrochim Acta. 2024 Oct 29;191(11):707. doi: 10.1007/s00604-024-06796-1.
An electrochemiluminescence (ECL) biosensor for carcinoembryonic antigen (CEA) based on electrochemiluminescence resonance energy transfer (ECL-RET) was designed. Tris(2,2'-bipyridine)ruthenium(II) (Ru(bpy)) was used as an energy donor for ECL-RET, and an Au nanoparticle-modified MOF framework (AuCoFe MOF) was used as an energy receptor for ECL-RET. The ECL emission spectra of Ru(bpy) were in the range 550 to 680 nm, and a zinc oxalate MOF encapsulating Ru(bpy) (Ru@ Zn oxalate MOF) was prepared. The UV-vis absorption spectrum of AuCoFe MOF ranges from 280 to 700 nm and overlaps with emission spectra of Ru@Zn oxalate MOF, which is critical for RET. The AuCoFe MOF-Ab bioconjugate, target CEA antigen, and the Ru@Zn oxalate MOF-Ab bioconjugate together form a sandwich structure, resulting in quenching of the ECL signal of Ru@Zn oxalate MOF by AuCoFe MOF. Under the optimized experimental conditions, the ECL-RET sensor exhibited excellent analytical performance in CEA detection with a linear range of 1.0 × 10 to 1.0 × 10 mg mL; the minimum limit of detection is 1.4 × 10 mg mL (S/N = 3); and its recoveries of spiked samples ranging from 99.1 to 100.7%. The developed sensor has excellent stability, reproducibility, and specificity and is suitable for the detection of CEA in human serum and has the potential to provide sensitive detection of other biomarkers of diseases.
基于电化学发光共振能量转移（ECL-RET）设计了一种用于癌胚抗原（CEA）的电化学发光（ECL）生物传感器。三（2,2'-联吡啶）钌（II）（Ru（bpy））被用作 ECL-RET 的能量供体，而金纳米粒子修饰的 MOF 框架（AuCoFe MOF）被用作 ECL-RET 的能量受体。Ru（bpy）的 ECL 发射光谱在 550 至 680nm 范围内，并且制备了包封 Ru（bpy）的草酸锌 MOF（Ru@Zn 草酸 MOF）。AuCoFe MOF 的紫外-可见吸收光谱范围为 280 至 700nm，并与 Ru@Zn 草酸 MOF 的发射光谱重叠，这对于 RET 至关重要。AuCoFe MOF-Ab 缀合物、靶标 CEA 抗原和 Ru@Zn 草酸 MOF-Ab 缀合物一起形成三明治结构，导致 Ru@Zn 草酸 MOF 的 ECL 信号被 AuCoFe MOF 猝灭。在优化的实验条件下，ECL-RET 传感器在 CEA 检测中表现出优异的分析性能，线性范围为 1.0×10 至 1.0×10 mg mL；最低检测限为 1.4×10 mg mL（S/N=3）；并且其从 99.1 到 100.7%的加标样品的回收率。所开发的传感器具有出色的稳定性、重现性和特异性，适用于人血清中 CEA 的检测，并且有可能提供对其他疾病生物标志物的敏感检测。
