Department of Ophthalmology, China Medical University Hospital, Taichung, Taiwan, R.O.C.
Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C.
Anticancer Res. 2024 Nov;44(11):4825-4831. doi: 10.21873/anticanres.17308.
BACKGROUND/AIM: The dysregulation of matrix metalloproteinase (MMP) proteins has been reported to be involved in the etiology of pterygium. However, studies about the role of matrix metalloproteinase-11 (MMP-11) are lacking. This study is the first to examine the genomic role of MMP-11 in pterygium.
The genotypes of MMP-11 rs738791, rs2267029, rs738792, and rs28382575 were determined in 140 pterygium cases and 280 non-pterygium controls by utilizing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing.
The genotypic frequencies of MMP-11 rs738792 TT, CT and CC were 40.0%, 50.7%, and 9.3% in the pterygium group, significantly different from those in the non-pterygium group (55.0%, 37.1%, and 7.9%, respectively; p for trend=0.0139). Specifically, individuals carrying the variants CT and CC had a 1.88- and 1.63-fold odds ratio of pterygium risk [95% confidence interval (CI)=1.22-2.89 and 0.77-3.44, p=0.0054 and 0.2834, respectively]. In the dominant model, individuals carrying CT+CC had significantly higher pterygium risk (odds ratio=1.83, 95%CI=1.21-2.77, p=0.0052). No association was found for other MMP-11 polymorphisms. Allelic analysis showed that MMP-11 rs738792 C allele was significantly associated with pterygium risk (odds ratio=1.48, 95%CI=1.08-2.01, p=0.0169). for the variant alleles of other MMP-11 polymorphisms were not associated with pterygium risk.
The MMP-11 rs738792 genotypes can serve as a predictive marker for pterygium risk in Taiwanese. Additionally, elucidating the role of MMP-11 in the pathogenesis of pterygium could inform targeted therapies based on MMP-11 modulation.
背景/目的：基质金属蛋白酶（MMP）蛋白的失调已被报道与翼状胬肉的病因有关。然而，关于基质金属蛋白酶 11（MMP-11）的作用的研究尚缺乏。本研究首次探讨了 MMP-11 在翼状胬肉中的基因组作用。
利用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和直接测序法，在 140 例翼状胬肉病例和 280 例非翼状胬肉对照中确定 MMP-11 rs738791、rs2267029、rs738792 和 rs28382575 的基因型。
翼状胬肉组中 MMP-11 rs738792 TT、CT 和 CC 的基因型频率分别为 40.0%、50.7%和 9.3%，明显不同于非翼状胬肉组（分别为 55.0%、37.1%和 7.9%；趋势检验 p=0.0139）。具体来说，携带 CT 和 CC 变异体的个体发生翼状胬肉的风险分别增加 1.88 倍和 1.63 倍[95%置信区间（CI）=1.22-2.89 和 0.77-3.44，p=0.0054 和 0.2834]。在显性模型中，携带 CT+CC 的个体发生翼状胬肉的风险显著升高（优势比=1.83，95%CI=1.21-2.77，p=0.0052）。其他 MMP-11 多态性无相关性。等位基因分析表明，MMP-11 rs738792 C 等位基因与翼状胬肉风险显著相关（优势比=1.48，95%CI=1.08-2.01，p=0.0169）。其他 MMP-11 多态性的变异等位基因与翼状胬肉风险无关。
MMP-11 rs738792 基因型可作为台湾人翼状胬肉风险的预测标志物。此外，阐明 MMP-11 在翼状胬肉发病机制中的作用，可以为基于 MMP-11 调节的靶向治疗提供信息。
