从胶质母细胞瘤患者血浆中提取的细胞外囊泡的诊断潜力。
The Diagnostic Potential of Extracellular Vesicles Derived From the Blood Plasma of Glioblastoma Patients.
机构信息
Department of Diagnostic and Interventional Neuroradiology, Medical School Hannover, Hannover, Germany.
Department of Neurosurgery, University Medical Center Goettingen, Goettingen, Germany.
出版信息
In Vivo. 2024 Nov-Dec;38(6):2735-2739. doi: 10.21873/invivo.13752.
BACKGROUND/AIM: Biomarkers for patients suffering from glioblastoma (GBM) are scarce. Extracellular vesicles (EV) are a promising candidate for a potential biomarker. Therefore, EV concentration could be a potential biomarker of tumor burden, volume, and prognosis.
PATIENTS AND METHODS
Large EV (lEV) and small EV (sEV) were isolated from 36 GBM patients' blood plasma by differential centrifugation. Nanoparticle tracking was used to measure EV concentration. Quantitative analysis of tumor volume was performed by evaluating T2/FLAIR relaxation times.
RESULTS
The mean size of lEV was 173.3 nm ± 18.2 nm, while sEV measured 148.3 ± 9.0 nm. Patients with higher lEV concentrations showed a trend towards longer overall survival (36.1 vs. 16.5 months, p=0.08). Regarding inflammatory markers, higher leukocyte count was positively correlated with higher sEV concentration (r=0.3887, DF 21, p=0.0015). No significant relationship was found between lEV or sEV concentration and tumor volume.
CONCLUSION
Overall EV concentration in the peripheral blood is not a predictor of tumor volume. sEV concentration is associated with a potential pro-inflammatory metabolism.
背景/目的：患有胶质母细胞瘤（GBM）的患者的生物标志物稀缺。细胞外囊泡（EV）是一种有前途的潜在生物标志物候选物。因此，EV 浓度可能是肿瘤负担、体积和预后的潜在生物标志物。
患者和方法
通过差速离心从 36 名 GBM 患者的血浆中分离出大细胞外囊泡（lEV）和小细胞外囊泡（sEV）。使用纳米颗粒跟踪技术测量 EV 浓度。通过评估 T2/FLAIR 弛豫时间来定量分析肿瘤体积。
结果
lEV 的平均大小为 173.3nm±18.2nm，而 sEV 为 148.3nm±9.0nm。lEV 浓度较高的患者总生存期呈延长趋势（36.1 个月与 16.5 个月，p=0.08）。关于炎症标志物，白细胞计数越高，sEV 浓度越高（r=0.3887，DF 21，p=0.0015）。未发现 lEV 或 sEV 浓度与肿瘤体积之间存在显著关系。
结论
外周血中总的 EV 浓度不是肿瘤体积的预测因子。sEV 浓度与潜在的促炎代谢有关。
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