Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, P.R. China.
Yunnan Key Laboratory of Oral Medicine, Kunming, P.R. China.
In Vivo. 2024 Nov-Dec;38(6):2665-2671. doi: 10.21873/invivo.13743.
BACKGROUND/AIM: To investigate the feasibility of establishing a mandibular osteosarcoma model in Sprague-Dawley (SD) rats using tissue block transplantation, providing a foundational model for osteosarcoma research.
Fourteen male SD rats, 3 weeks old and SPF grade, were randomly divided into a control group (n=4) and a mandibular osteosarcoma group (n=10). Using tissue block transplantation, UMR106 cell-induced tumor tissues were transplanted subcutaneously into the left mandibular marrow cavity of the SD rats. Observations included behavioral changes, weight variations, tumor growth, and tumor formation rate. Bone changes were monitored via micro-CT scanning, and histological analysis was conducted using HE staining.
Two weeks post-transplantation, the mandibular osteosarcoma group exhibited significant left facial swelling, malocclusion, eating difficulties, and weight loss compared to the control group. The tumor formation rate was 80% (8/10). Micro-CT scans indicated significant bone destruction in the osteosarcoma group. HE staining revealed high cellular atypia and pathological mitoses in both subcutaneous and mandibular osteosarcoma cells, with no notable abnormalities in lung tissues.
Tissue block transplantation is a viable method to establish a mandibular osteosarcoma model in SD rats. This method is simple, with a high tumor formation rate, providing an ideal animal model for mandibular osteosarcoma research.
背景/目的：通过组织块移植，探索在 Sprague-Dawley（SD）大鼠中建立下颌骨肉瘤模型的可行性，为骨肉瘤研究提供基础模型。
将 14 只 3 周龄 SPF 级雄性 SD 大鼠随机分为对照组（n=4）和下颌骨肉瘤组（n=10）。采用组织块移植，将 UMR106 细胞诱导的肿瘤组织移植到 SD 大鼠左侧下颌骨髓腔内。观察行为变化、体重变化、肿瘤生长和肿瘤形成率。通过 micro-CT 扫描监测骨变化，并通过 HE 染色进行组织学分析。
移植后 2 周，下颌骨肉瘤组与对照组相比，左侧面部肿胀、咬合不正、进食困难和体重减轻明显。肿瘤形成率为 80%（8/10）。micro-CT 扫描显示骨肉瘤组有明显的骨破坏。HE 染色显示皮下和下颌骨肉瘤细胞的细胞异型性高，病理性有丝分裂明显，肺组织无明显异常。
组织块移植是在 SD 大鼠中建立下颌骨肉瘤模型的一种可行方法。该方法简单，肿瘤形成率高，为下颌骨肉瘤研究提供了理想的动物模型。
