Wang Jiao, Huang Zhenwu, Li Simeng, Lv Zengpeng
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Biol Trace Elem Res. 2024 Oct 30. doi: 10.1007/s12011-024-04430-y.
Maternal nutrition plays an important role in regulating the growth and development of offspring. Collagen peptide chelated trace elements (PTE), as a new additive, have been proven to have a positive maternal effect on the intestinal health of offspring, but its effect on the growth and development is still unclear. This study aimed to investigate the effect of maternal PTE supplementation on the skeletal muscle development of offspring. A total of 270 breeder hens were randomly divided into 3 groups, and fed basal diet (CON), basal diet + 500 mg/kg PTE (L-PTE), and 1000 mg/kg PTE (H-PTE) for 8 weeks. A total of 180 eggs were collected from each group for incubation, and then the hatched male chicks (6 replicates, 12 chicks/replicate) were allocated according to maternal treatment for a 14-day feeding experiment. The results showed that maternal PTE supplementation significantly increased the deposition of iron (Fe), zinc (Zn), and manganese (Mn) in the egg yolk (P < 0.05). In comparison with the CON group, the body weight (days 1 and 14), breast muscle weight (day 14), and muscle fiber density (day 14) of broilers were increased in the L-PTE group (P < 0.05). The serum creatinine (CREA) levels in 1-day-old broilers were reduced in the L-PTE group and H-PTE group (P < 0.05). Additionally, maternal PTE supplementation could upregulate the relative expression level of catalase (CAT) mRNA in breast muscle (P < 0.05). The relative mRNA levels of hemeoxygenase-1 (HO-1) and superoxide dismutase 1(SOD1) in the H-PTE group were significantly upregulated (P < 0.05). Moreover, PTE treatment upregulated the mRNA expression of skeletal muscle development-related genes (Pax7 and MyoG) and the IGF signaling pathway (mTOR, IGF-1R, and IGF-2R) (P < 0.05). In conclusion, maternal PTE supplementation may improve the growth performance and skeletal muscle development of offspring by activating the IGF signaling pathway.
母体营养在调节后代的生长发育中起着重要作用。胶原蛋白肽螯合微量元素（PTE）作为一种新型添加剂，已被证明对后代的肠道健康具有积极的母体效应，但其对生长发育的影响仍不清楚。本研究旨在探讨母体补充PTE对后代骨骼肌发育的影响。将270只种母鸡随机分为3组，分别饲喂基础日粮（CON）、基础日粮+500 mg/kg PTE（L-PTE）和1000 mg/kg PTE（H-PTE），持续8周。每组收集180枚鸡蛋用于孵化，然后将孵化出的雄性雏鸡（6个重复，每个重复12只雏鸡）根据母体处理方式进行分配，进行为期14天的饲养实验。结果表明，母体补充PTE显著增加了蛋黄中铁（Fe）、锌（Zn）和锰（Mn）的沉积（P<0.05）。与CON组相比，L-PTE组肉鸡的体重（第1天和第14天）、胸肌重量（第14天）和肌纤维密度（第14天）均有所增加（P<0.05）。L-PTE组和H-PTE组1日龄肉鸡的血清肌酐（CREA）水平降低（P<0.05）。此外，母体补充PTE可上调胸肌中过氧化氢酶（CAT）mRNA的相对表达水平（P<0.05）。H-PTE组中血红素加氧酶-1（HO-1）和超氧化物歧化酶1（SOD1）的相对mRNA水平显著上调（P<0.05）。此外，PTE处理上调了骨骼肌发育相关基因（Pax7和MyoG）以及IGF信号通路（mTOR、IGF-1R和IGF-2R）的mRNA表达（P<0.05）。总之，母体补充PTE可能通过激活IGF信号通路来改善后代的生长性能和骨骼肌发育。
