State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Aksu Vocational and Technical College, Aksu, China.
J Sci Food Agric. 2024 Jan 15;104(1):174-183. doi: 10.1002/jsfa.12938. Epub 2023 Sep 12.
Dietary supplementation with trace elements zinc (Zn), iron (Fe) and manganese (Mn) could promote intestinal development and improve intestinal health. There are, however, few studies examining the possibility that maternal original Zn, Fe and Mn could regulate intestinal development and barrier function in the offspring. This study aimed to investigate how the intestinal growth and barrier function of breeder offspring were affected by collagen peptide-chelated trace elements (PTE; Zn, Fe, Mn).
PTE supplementation in the diet of breeder hens increased the concentrations of Zn, Fe and Mn in egg yolk. Maternal PTE supplementation improved morphological parameters of the intestine (villi height, crypt depth and villi height/crypt depth) and upregulated the mRNA expression level of leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) in the ileum of chick embryos. Furthermore, maternal PTE effect improved villi height/crypt depth of offspring at 1 and 14 days of age, and upregulated Lgr5, Claudin-3 and E-cadherin mRNA expression in the broiler ileum. Additionally, PTE treatment could enhance the intestinal microbial diversity of offspring. Maternal PTE supplementation increased the relative abundance of Clostridiales at the genus level and decreased the relative abundance of Enterococcus in newborn offspring. Moreover, maternal PTE supplementation ameliorated the elevated nuclear factor kappa B, toll-like receptor 4 and interleukin 1β mRNA expression in the ileum of offspring caused by LPS challenge.
Maternal PTE supplementation could promote intestinal development and enhance the intestinal barrier function of chicken offspring. © 2023 Society of Chemical Industry.
微量元素锌（Zn）、铁（Fe）和锰（Mn）的膳食补充剂可以促进肠道发育并改善肠道健康。然而，很少有研究探讨母体原始 Zn、Fe 和 Mn 是否可以调节后代的肠道发育和屏障功能。本研究旨在探讨育雏鸡的肠道生长和屏障功能如何受到胶原蛋白肽螯合微量元素（PTE；Zn、Fe、Mn）的影响。
日粮中添加 PTE 可提高蛋黄中 Zn、Fe 和 Mn 的浓度。母体 PTE 补充剂改善了鸡胚回肠的形态学参数（绒毛高度、隐窝深度和绒毛高度/隐窝深度），并上调了 Lgr5 的 mRNA 表达水平。此外，母体 PTE 作用改善了 1 和 14 日龄雏鸡的绒毛高度/隐窝深度，并上调了肉鸡回肠中 Lgr5、Claudin-3 和 E-钙粘蛋白的 mRNA 表达。此外，PTE 处理可以增强后代的肠道微生物多样性。母体 PTE 补充剂增加了属水平上梭菌的相对丰度，并降低了新生后代肠球菌的相对丰度。此外，母体 PTE 补充剂可改善 LPS 刺激引起的雏鸡回肠中核因子 kappa B、Toll 样受体 4 和白细胞介素 1β mRNA 表达的升高。
母体 PTE 补充剂可促进鸡后代的肠道发育并增强其肠道屏障功能。
