Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China.
Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark.
Sci Data. 2024 Oct 30;11(1):1175. doi: 10.1038/s41597-024-03843-7.
The diatom Paralia guyana is a tychoplanktonic microalgal species that represents one of the early diverging diatoms. P. guyana can thrive in both planktonic and benthic habitats, making a significant contribution to the occurrence of red tide events. Although a dozen diatom genomes have been sequenced, the identity of the early diverging diatoms remains elusive. The understanding of the evolutionary clades and mechanisms of ecological adaptation in P. guyana is limited by the absence of a high-quality genome assembly. In this study, the first high-quality genome assembly for the early diverging diatom P. guyana was established using PacBio single molecular sequencing. The assembled genome has a size of 558.85 Mb, making it the largest diatom genome on record, with a contig N50 size of 26.06 Mb. A total of 27,121 protein-coding genes were predicted in the P. guyana genome, of which 22,904 predicted genes (84.45%) were functionally annotated. This data and analysis provide innovative genomic resources for tychoplanktonic microalgal species and shed light on the evolutionary origins of diatoms.
盘状硅藻 Paralia guyana 是一种浮游微藻物种，代表了早期分化的硅藻之一。P. guyana 可以在浮游和底栖生境中茁壮成长，对赤潮事件的发生做出了重要贡献。尽管已经测序了十几个硅藻基因组，但早期分化的硅藻的身份仍然难以捉摸。由于缺乏高质量的基因组组装，对 P. guyana 中进化枝系和生态适应机制的理解受到限制。在这项研究中，使用 PacBio 单分子测序为早期分化的硅藻 P. guyana 建立了第一个高质量的基因组组装。组装的基因组大小为 558.85 Mb，是记录中最大的硅藻基因组，其重叠群 N50 大小为 26.06 Mb。在 P. guyana 基因组中预测了总共 27121 个蛋白编码基因，其中 22904 个预测基因（84.45%）具有功能注释。这些数据和分析为浮游微藻物种提供了创新性的基因组资源，并揭示了硅藻的进化起源。
