Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, Jiangsu, China.
Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Wuxi, 214081, Jiangsu, China.
Sci Data. 2024 Oct 30;11(1):1177. doi: 10.1038/s41597-024-04025-1.
The cold stress during overwintering is considered the bottleneck of red tilapia industry. In this study, the water temperature (WT) was reduced by 2 °C per day from 20 °C to 8 °C in the cold (C) group. Then transcriptome of brain(B), gill(G), liver(L) and skin(S) tissues and proteome of G, L and S tissues were performed in C and Normal (N) (WT: 20 °C) group. 24 transcriptomes were completed, and 168.8 Gb data were obtained, with more than 5.89 Gb clean data of each sample. A total of 30499 annotation results were obtained with 3199, 4697, 4393, and 3382 differentially expressed mRNAs in NB_vs_CB, NG_vs_CG, NL_vs_CL, NS_vs_CS. 18 DIA proteomes were performed, and 6341 proteins were obtained with 178, 500 and 166 differentially expressed proteins in NG_vs_CG, NL_vs_CL, NS_vs_CS. Our datasets can be reused for key genes and proteins identification, omics joint analysis and regulatory mechanism analysis of low temperature or cold stress in fish, which will help understanding the regulatory mechanism and facilitate the molecular selective breeding of cold-resistant varieties of fish.
越冬期间的冷应激被认为是红罗非鱼产业的瓶颈。在这项研究中，冷水（C）组的水温（WT）每天从 20°C 降低 2°C 至 8°C。然后在 C 和正常（N）（WT：20°C）组中对脑（B）、鳃（G）、肝（L）和皮肤（S）组织进行转录组分析，对 G、L 和 S 组织进行蛋白质组分析。完成了 24 个转录组，获得了 168.8Gb 的数据，每个样本的清洁数据超过 5.89Gb。总共获得了 30499 个注释结果，NB_vs_CB、NG_vs_CG、NL_vs_CL、NS_vs_CS 中分别有 3199、4697、4393 和 3382 个差异表达的 mRNAs。进行了 18 个 DIA 蛋白质组学分析，获得了 6341 个蛋白质，NG_vs_CG、NL_vs_CL、NS_vs_CS 中分别有 178、500 和 166 个差异表达的蛋白质。我们的数据集可重复用于鉴定关键基因和蛋白质、组学联合分析和鱼类低温或冷应激的调控机制分析，这将有助于理解调控机制，并促进鱼类耐寒品种的分子选择育种。
